1.
What is the primary function of CRISPR-Cas9?
Correct Answer
B. Binding to target DNA and cleaving it
Explanation
CRISPR-Cas9 is an enzyme that plays a pivotal role in genome editing. It works by binding to specific target DNA sequences guided by a guide RNA (gRNA). Once bound, CRISPR-Cas9 cleaves the DNA at that precise location, allowing for targeted genetic modifications. This precision in DNA editing has revolutionized biotechnology and medical research, enabling scientists to edit genes with unprecedented accuracy. The ability to modify DNA sequences has wide-ranging implications, from correcting genetic mutations associated with hereditary diseases to engineering crops for improved agricultural yield.
2.
Which of the following is a biopharmaceutical product?
Correct Answer
A. Insulin
Explanation
Insulin is a hormone produced by the pancreas that regulates blood sugar levels in the body. In biotechnology, insulin is synthesized using genetically engineered bacteria or yeast cells. This process, known as recombinant DNA technology, involves inserting the gene encoding human insulin into the DNA of host organisms. The host cells then produce insulin, which can be harvested and purified for medical use. Insulin produced through biotechnology is identical to naturally occurring human insulin and has revolutionized the treatment of diabetes, providing a safe and effective way to manage blood sugar levels in individuals with diabetes.
3.
What is recombinant DNA technology commonly used for?
Correct Answer
A. Vaccine production
Explanation
Recombinant DNA technology is a fundamental tool in biotechnology that involves combining DNA from different sources to create new genetic combinations. One of its most common applications is in vaccine production. By introducing genes encoding antigens from pathogens into host organisms, scientists can stimulate an immune response without causing disease. This approach has led to the development of vaccines against a wide range of infectious diseases, including hepatitis B, human papillomavirus (HPV), and COVID-19. Recombinant DNA technology has revolutionized vaccine production, enabling the rapid development of vaccines with improved safety and efficacy.
4.
Which technology allows precise modification of genetic code?
Correct Answer
C. CRISPR-Cas9
Explanation
CRISPR-Cas9 is a revolutionary gene editing technology that allows precise modifications to the genetic code. It works by guiding the Cas9 enzyme to specific DNA sequences using a guide RNA (gRNA). Once bound, Cas9 induces double-strand breaks in the DNA, which can be repaired by cellular mechanisms to introduce desired genetic changes. CRISPR-Cas9 has transformed biotechnology and medical research by providing a versatile tool for editing genes in a wide range of organisms. Its applications include correcting genetic mutations associated with hereditary diseases, engineering crops for improved yield and resistance to pests, and studying the function of genes in model organisms.
5.
What is a common application of biotechnology in regenerative medicine?
Correct Answer
A. Tissue engineering
Explanation
Tissue engineering is a multidisciplinary field that combines principles of biology, engineering, and materials science to create functional tissues or organs for medical purposes. It involves seeding cells onto scaffolds and providing the appropriate biochemical and physical cues to promote tissue growth and maturation. Tissue engineering holds promise for regenerative medicine, offering potential solutions for organ transplantation, tissue repair, and disease modeling. By harnessing the body's natural regenerative capacity and combining it with advances in biotechnology, tissue engineers aim to develop therapies that can restore function to damaged or diseased tissues.
6.
Which biotech tool enables targeted editing of genes?
Correct Answer
D. CRISPR-Cas9
Explanation
CRISPR-Cas9 is a powerful gene editing tool that enables targeted modifications to the genome. It works by guiding the Cas9 enzyme to specific DNA sequences using a guide RNA (gRNA). Once bound, Cas9 induces double-strand breaks in the DNA, which can be repaired by cellular mechanisms to introduce desired genetic changes. CRISPR-Cas9 has revolutionized biotechnology and medical research by providing a versatile platform for editing genes in a wide range of organisms. Its precision, efficiency, and ease of use have led to numerous applications, including gene therapy, drug discovery, and agricultural biotechnology.
7.
What type of molecules does CRISPR-Cas9 target for editing?
Correct Answer
B. DNA sequences
Explanation
CRISPR-Cas9 is a genome editing tool that targets specific DNA sequences for modification. It achieves this by binding to complementary DNA sequences guided by a guide RNA (gRNA) molecule. Once bound, the Cas9 enzyme induces site-specific cleavage or modification of the DNA, allowing for precise genetic alterations. CRISPR-Cas9 has emerged as a transformative technology in biotechnology and medical research, offering unprecedented control over gene expression and function. Its ability to edit genes with high precision has enabled a wide range of applications, from correcting genetic mutations associated with hereditary diseases to engineering crops for improved agricultural yield.
8.
Which biotech method is used in producing genetically engineered vaccines?
Correct Answer
A. Recombinant DNA technology
Explanation
Recombinant DNA technology is a cornerstone of modern biotechnology that allows scientists to manipulate the genetic material of organisms for various purposes. One of its key applications is in the production of genetically engineered vaccines. This process involves inserting genes encoding antigens from pathogens into host organisms, such as bacteria or yeast cells. The host cells then produce the antigens, which can stimulate an immune response without causing disease. Recombinant DNA technology has revolutionized vaccine production, enabling the rapid development of vaccines against infectious diseases like hepatitis B, human papillomavirus (HPV), and COVID-19.
9.
In personalized medicine, treatment plans are based on an individual's what?
Correct Answer
A. Genetic makeup
Explanation
Personalized medicine is an approach to healthcare that takes into account individual differences in genetics, environment, and lifestyle when designing treatment plans. By analyzing a patient's genetic makeup, healthcare providers can tailor medical interventions to match the specific needs of each individual. Personalized medicine holds promise for improving treatment outcomes, minimizing adverse reactions, and optimizing healthcare delivery. Genetic makeup plays a central role in personalized medicine, providing valuable insights into disease risk, treatment response, and drug metabolism.
10.
Which biotech innovation has revolutionized cancer treatment?
Correct Answer
C. Monoclonal antibodies
Explanation
Monoclonal antibodies are a class of biopharmaceuticals that have revolutionized the treatment of cancer. They work by targeting specific proteins involved in cancer growth and progression, blocking their activity and triggering immune responses against cancer cells. Monoclonal antibodies have become essential tools in cancer therapy, offering targeted treatment options with fewer side effects compared to traditional chemotherapy. Their ability to selectively target cancer cells while sparing healthy tissues has transformed the landscape of cancer treatment, improving survival rates and quality of life for patients.
11.
What is a common biotechnological approach to developing gene therapies?
Correct Answer
D. Gene editing
Explanation
Gene editing is a biotechnological approach used in developing gene therapies, which aim to treat or cure genetic diseases by modifying the patient's DNA. Gene editing technologies like CRISPR-Cas9 enable precise modifications to the genetic code, allowing scientists to correct or replace defective genes associated with genetic disorders. Gene editing holds promise for treating a wide range of genetic diseases, including cystic fibrosis, sickle cell anemia, and Duchenne muscular dystrophy. By harnessing the power of biotechnology to edit genes, researchers are paving the way for innovative therapies that could transform the lives of patients with genetic disorders.
12.
Which biotech tool allows the amplification of specific DNA sequences?
Correct Answer
C. Polymerase Chain Reaction
Explanation
Polymerase Chain Reaction (PCR) is a fundamental biotech tool used to amplify specific DNA sequences. It works by repeatedly heating and cooling a DNA sample to denature, anneal, and extend DNA strands, resulting in the exponential amplification of the target sequence. PCR has revolutionized molecular biology and biotechnology by providing a rapid and efficient method for copying DNA. Its applications include DNA sequencing, genetic testing, and forensic analysis. PCR has become indispensable in modern biotechnology, enabling researchers to analyze and manipulate DNA with unprecedented precision and speed.
13.
What type of cells are commonly used in tissue engineering?
Correct Answer
A. Stem cells
Explanation
Stem cells are undifferentiated cells with the unique ability to differentiate into various cell types and self-renew indefinitely. They play a crucial role in tissue engineering, a field of biotechnology focused on creating functional tissues or organs for medical purposes. Stem cells serve as building blocks for tissue regeneration, providing a source of cells for repairing or replacing damaged tissues and organs. Their versatility and regenerative potential make them valuable tools in regenerative medicine, offering promising solutions for treating injuries, degenerative diseases, and other medical conditions.
14.
Which biotech method is used to study gene expression?
Correct Answer
C. RNA sequencing
Explanation
RNA sequencing is a high-throughput biotech method used to study gene expression by measuring the abundance of RNA transcripts in a sample. It provides valuable insights into the activity of genes and their regulation under different conditions, allowing researchers to identify genes that are turned on or off in response to stimuli. RNA sequencing has revolutionized the field of genomics, offering a comprehensive view of gene expression patterns across the entire transcriptome. Its applications include disease research, drug discovery, and personalized medicine, providing valuable information for understanding the molecular mechanisms underlying health and disease.
15.
What is the primary purpose of using monoclonal antibodies?
Correct Answer
A. Targeting specific proteins
Explanation
Monoclonal antibodies are a class of biopharmaceuticals that are designed to target specific proteins involved in diseases. They work by binding to their target proteins with high specificity and affinity, triggering immune responses against pathogens or cancer cells. In cancer treatment, monoclonal antibodies are used to target proteins on the surface of cancer cells, blocking their growth and survival. They can also be conjugated with toxins or radioisotopes to deliver targeted therapies directly to cancer cells while sparing healthy tissues. Monoclonal antibodies have revolutionized cancer therapy, offering effective and targeted treatments with fewer side effects compared to traditional chemotherapy and radiation therapy.