1.
What is the primary goal of reproductive cloning?
Correct Answer
B. Producing genetically identical organisms
Explanation
Reproductive cloning, a complex biotechnological process, involves creating genetically identical organisms by mirroring the genetic composition of an existing individual. A nuanced exploration of reproductive cloning reveals its intricate mechanisms, ethical considerations, and potential applications across various fields of science and technology. The outcome of reproductive cloning is an organism that is genetically identical to the donor organism. This genetic identicalness extends to both nuclear and mitochondrial DNA, resulting in a nearly identical genetic makeup.
2.
Which cloning technique was used to create Dolly the sheep?
Correct Answer
A. Somatic Cell Nuclear Transfer (SCNT)
Explanation
Dolly, the groundbreaking cloned sheep born in 1996, was a product of Somatic Cell Nuclear Transfer (SCNT), a revolutionary technique that brought the concept of cloning to the forefront of scientific and public attention. Dolly was created using a mammary gland cell from a six-year-old Finn Dorset ewe as the donor cell. This somatic cell nucleus was fused with an enucleated egg from a Scottish Blackface sheep. The success of this process demonstrated the ability to reprogram a fully differentiated somatic cell to develop into a viable organism.
3.
What is a potential application of therapeutic cloning?
Correct Answer
B. Generating tissues for medical treatment
Explanation
Therapeutic cloning, a cutting-edge technology, is specifically designed to generate tissues and cells for medical treatments, holding immense promise in the field of regenerative medicine. Therapeutic cloning is a key component of regenerative medicine, a field focused on harnessing the body's natural healing abilities to repair or replace damaged tissues. The primary goal is to generate cells and tissues that can be used for medical treatments, offering novel approaches to treat a wide range of conditions.
4.
In the context of cloning, what is "embryo splitting"?
Correct Answer
B. Dividing a developing embryo into two
Explanation
Embryo splitting, a fascinating form of cloning, involves dividing a developing embryo into two parts, ultimately leading to the formation of identical twins or clones.
The embryo-splitting process typically occurs at the early stages of embryonic development, usually within the first few cell divisions. The procedure involves carefully separating the cells of the developing embryo to create two distinct entities. This manipulation results in two embryos, each carrying identical genetic information.The primary outcome of embryo splitting is the creation of genetically identical individuals. As each resulting embryo originates from the same initial fertilized egg, the genetic makeup of the clones is virtually identical. This makes embryo splitting a unique method of producing clones with shared genetic material.
5.
Which reproductive technology involves combining genetic material from multiple sources?
Correct Answer
A. Hybridization
Explanation
The reproductive technology that involves combining genetic material from multiple sources is “Hybridization”. Hybridization in biology is the process of combining different varieties of organisms to create a hybrid. This process can occur naturally through sexual reproduction, where genetic material from two parents is combined to produce offspring with a mix of traits from both parents. In the context of reproductive technologies, hybridization can also be achieved artificially, for example, by manually cross-pollinating plants to combine desirable traits. This technology has been used extensively in agriculture to improve crop varieties. It’s important to note that while other technologies like IVF, SCNT, and CRISPR-Cas9 involve manipulation of genetic material, they do not typically involve combining genetic material from multiple sources.
6.
What is the primary challenge associated with reproductive cloning?
Correct Answer
D. Limited success in achieving viable clones
Explanation
Reproductive cloning, a complex process aiming to produce genetically identical organisms, confronts a multitude of challenges rooted in the limited success of achieving viable clones. One of the primary challenges in reproductive cloning is the consistently low success rates. Many attempts to produce viable clones result in failure, with only a fraction of cloned embryos developing into healthy organisms. The inefficiency of the process poses a significant obstacle to the widespread adoption of cloning technologies. Cloned organisms often exhibit developmental abnormalities. These abnormalities can manifest in various ways, affecting the structure and function of organs leading to health issues in the cloned individuals. The incidence of abnormalities underscores the complexity of recreating normal embryonic development through cloning.
7.
What is the main purpose of pre-implantation genetic diagnosis (PGD)?
Correct Answer
A. Determining genetic abnormalities
Explanation
Pre-implantation Genetic Diagnosis (PGD) stands as a pivotal and sophisticated tool in the realm of assisted reproductive technologies, particularly during in vitro fertilization (IVF). PGD involves the analysis of genetic material from embryos created through IVF before their implantation in the uterus. Typically conducted at the blastocyst stage (around day 5-7 post-fertilization), a small number of cells are biopsied from each embryo. Various molecular techniques, such as polymerase chain reaction (PCR) and next-generation sequencing, are then employed to analyze the genetic information.
8.
Which gene-editing technology is widely used in reproductive research?
Correct Answer
B. CRISPR-Cas9
Explanation
CRISPR-Cas9, a revolutionary gene-editing technology, has significantly transformed the landscape of reproductive research, providing an unparalleled tool for targeted genetic modifications. CRISPR-Cas9 utilizes a naturally occurring bacterial defense system. The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) are specific DNA sequences in bacteria that retain information about viral DNA. The CRISPR-associated protein 9 (Cas9) acts as molecular scissors, guided by RNA molecules, to cut and edit specific DNA sequences precisely. One of the hallmark features of CRISPR-Cas9 is its unparalleled precision in targeting and modifying specific genes. Researchers design guide RNA sequences complementary to the target gene, directing Cas9 to the precise location in the genome. This targeted approach enables the addition, deletion, or replacement of genetic material with unprecedented accuracy.
9.
What is "parthenogenesis" in the context of reproductive technologies?
Correct Answer
B. Artificial activation of an unfertilized egg
Explanation
Parthenogenesis, a fascinating reproductive phenomenon, refers to the development of an organism from an unfertilized egg, often activated artificially in some cases.
Parthenogenesis occurs when an egg develops into an organism without fertilization by sperm. In certain cases, activation mechanisms, such as chemical or physical stimuli, can trigger the egg to initiate development. The absence of genetic material from a male gamete results in offspring that are genetically identical or closely related to the mother. Parthenogenesis has practical applications in biotechnology. In laboratory settings, researchers can artificially activate unfertilized eggs through various methods, including electrical or chemical stimulation. This controlled activation allows for the generation of embryos without the need for fertilization, providing a valuable tool for studies in developmental biology and genetic research.
10.
What ethical considerations are associated with reproductive cloning?
Correct Answer
C. Social and cultural implications
Explanation
The ethical considerations entwined with reproductive cloning transcend the boundaries of scientific inquiry, delving into complex social and cultural impact realms.
Ethical concerns extend to the potential disparities in access to cloning technologies. Questions arise about the socio-economic implications of cloning, including the accessibility of these technologies to different segments of society. Debates explore the risk of exacerbating existing social inequalities and emphasize the importance of equitable distribution and responsible application of cloning technologies.