Quiz: How Good Are You at Using a Micropipette?
Micropipettes are essential tools in the lab, but how skilled are you at using them? Take our Micropipette Quiz and gauge your precision! This quiz is designed to evaluate your knowledge of micropipettes, their parts, and their uses. Navigate through questions testing your understanding of volumes, calibration, and proper handling.
Whether you're a seasoned scientist or a novice, this quiz will challenge your micropipetting prowess. Assess your pipetting finesse and uncover any areas that may need improvement. Ready to prove your proficiency with a micropipette? Dive into the Micropipette Quiz now and see if you can pipette your way to the Read moretop!
Micropipette Usage Questions and Answers
- 1.
What is the purpose of a micropipette?
- A.
To measure small volumes of liquid
- B.
To transfer large volumes of liquid
- C.
To mix solutions together
- D.
To heat samples
Correct Answer
A. To measure small volumes of liquidExplanation
A micropipette is used to accurately measure and transfer small volumes of liquid, typically ranging from 0.1 μL to 1000 μL. It is commonly used in laboratories for various scientific and research purposes.Rate this question:
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- 2.
Which part of a micropipette is responsible for selecting the desired volume?
- A.
Plunger
- B.
Tip ejector
- C.
Volume adjustment dial
- D.
Shaft
Correct Answer
C. Volume adjustment dialExplanation
The volume adjustment dial is the part of a micropipette that allows the user to select the desired volume. By turning the dial, the user can set the micropipette to aspirate and dispense the required volume with high precision.Rate this question:
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- 3.
What is the purpose of the plunger in a micropipette?
- A.
To eject the pipette tip
- B.
To adjust the volume
- C.
To release the liquid
- D.
To sterilize the micropipette
Correct Answer
C. To release the liquidExplanation
The plunger in a micropipette is used to release the liquid once the desired volume has been aspirated. By pressing the plunger, the liquid is dispensed accurately and precisely, allowing for controlled transfer of the liquid to the desired location.Rate this question:
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- 4.
What is the maximum volume a P200 micropipette can measure?
- A.
200 μL
- B.
20 μL
- C.
1000 μL
- D.
5000 μL
Correct Answer
A. 200 μLExplanation
A P200 micropipette is designed to measure a maximum volume of 200 μL. It is commonly used for medium volume transfers and is suitable for applications that require precise and accurate pipetting within this range.Rate this question:
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- 5.
Which technique should be used while pipetting to ensure accuracy?
- A.
Rapid pipetting
- B.
Vertical angle pipetting
- C.
Not using pipette tips
- D.
Using a large dropper for measurement
Correct Answer
B. Vertical angle pipettingExplanation
Vertical angle pipetting is the recommended technique for ensuring accuracy while pipetting. Holding the micropipette vertically and at a slight angle, touching the inner wall of the receiving vessel, helps prevent sample loss, air bubbles, and ensures precise measurement.Rate this question:
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- 6.
How should a micropipette be stored when not in use?
- A.
Plunger fully depressed
- B.
Volume dial set to maximum
- C.
Standing upright
- D.
Left in a liquid-filled container
Correct Answer
C. Standing uprightExplanation
A micropipette should be stored standing upright when not in use. This prevents any liquid inside the micropipette from flowing back into the internal mechanism, which could cause damage or malfunction. Additionally, it helps maintain the cleanliness and integrity of the micropipette.Rate this question:
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- 7.
What should be done if a micropipette tip is stuck or difficult to eject?
- A.
Continue using force until it ejects
- B.
Disassemble the micropipette to remove the tip
- C.
Tap the side of the micropipette on a hard surface
- D.
Ignore it and proceed with pipetting
Correct Answer
C. Tap the side of the micropipette on a hard surfaceExplanation
If a micropipette tip is stuck or difficult to eject, one should tap the side of the micropipette gently on a hard surface. This helps dislodge the tip and allows for easy ejection without the risk of damaging the micropipette or creating an aerosol of the sample droplet.Rate this question:
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- 8.
What should be done if liquid comes in contact with the volume adjustment dial?
- A.
Continue using the micropipette as normal
- B.
Immediately clean and dry the volume adjustment dial
- C.
Dispose of the micropipette and get a new one
- D.
Place the micropipette in a warm oven to evaporate the liquid
Correct Answer
B. Immediately clean and dry the volume adjustment dialExplanation
If liquid comes in contact with the volume adjustment dial, it should be immediately cleaned and dried. Liquid on the dial may affect the accuracy of the micropipette's volume setting and potentially cause malfunction. Cleaning ensures reliable and precise measurements can be made.Rate this question:
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- 9.
Which of the following is a maintenance practice for micropipettes?
- A.
Regularly lubricating the plunger
- B.
Storing micropipettes in direct sunlight
- C.
Autoclaving a micropipette with the tip attached
- D.
Using harsh chemicals for cleaning
- E.
None of the above
Correct Answer
E. None of the aboveExplanation
Maintenance practices for micropipettes do not include regularly lubricating the plunger, storing micropipettes in direct sunlight, autoclaving with the tip attached, or using harsh chemicals for cleaning. These practices can lead to damage, inaccurate measurements, or contamination. Proper handling, routine calibration, and cleaning with mild solutions are recommended instead.Rate this question:
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- 10.
In which situations should a new micropipette tip be used?
- A.
After transferring a large volume
- B.
When changing to a different liquid or sample
- C.
If the micropipette has been dropped or mishandled
- D.
All of the above
Correct Answer
D. All of the aboveExplanation
A new micropipette tip should be used after transferring a large volume, when changing to a different liquid or sample, or if the micropipette has been dropped or mishandled. Using a new tip in these situations ensures accuracy, prevents cross-contamination, and maintains the integrity of the samples being handled.Rate this question:
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Quiz Review Timeline +
Our quizzes are rigorously reviewed, monitored and continuously updated by our expert board to maintain accuracy, relevance, and timeliness.
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Current Version
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Nov 17, 2023Quiz Edited by
ProProfs Editorial Team -
Nov 16, 2023Quiz Created by
Smriti Singh
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