Culture Media For Bacteriology

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Andrew Blanshard
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Quizzes Created: 9 | Total Attempts: 41,100
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  • 1/16 Questions

    Chococlate agar is made from blood agar

    • True
    • False
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About This Quiz

Bacteriology is the branch of biology that studies the morphology, ecology, genetics and biochemistry of bacteria. To figure out how much information you know about the culture media for bacteriology, take this quiz. All the best.

Culture Media For Bacteriology - Quiz

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  • 2. 

    MacConkey agar contains bile salts, peptones and lactose.

    • True

    • False

    Correct Answer
    A. True
    Explanation
    MacConkey agar is a selective and differential medium used in microbiology to isolate and differentiate gram-negative bacteria, particularly those of the Enterobacteriaceae family. The agar contains bile salts, which inhibit the growth of gram-positive bacteria, and peptones, which provide nutrients for the growth of gram-negative bacteria. Additionally, MacConkey agar contains lactose as a carbohydrate source. The ability of bacteria to ferment lactose is indicated by a color change in the agar, allowing for the differentiation of lactose-fermenting (pink/red colonies) and non-lactose fermenting (colorless colonies) bacteria. Therefore, the statement that MacConkey agar contains bile salts, peptones, and lactose is true.

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  • 3. 

    CLED agar is often used for culture of urine?

    • True

    • False

    Correct Answer
    A. True
    Explanation
    Cystine, lactose electrolyte deficient agar

    Stops proteus swarming due to absence of electrolytes.

    Can be used to indicate lactose fermentation if bromothylmol blue is added.

    Often used in the culture of urine.

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  • 4. 

    Which agar is selective for Vibrio spp?

    • Sabouraud Dextrose

    • XLD

    • TCBS

    • MacConkey

    Correct Answer
    A. TCBS
    Explanation
    Thiosulphate, citrate, bile salt agar.

    Also contains sucrose and thymol blue.

    Is selective for Vibrio spp.

    Vibrio cholerae ferments sucrose and produces yellow colonies.

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  • 5. 

    Vibrio cholera ferments sucrose and turns the media yellow on which agar?

    • MacConkey

    • XLD

    • Chocolate

    • TCBS

    Correct Answer
    A. TCBS
    Explanation
    Vibrio cholerae is a bacterium that causes cholera. It is known to ferment sucrose, which means it can break down sucrose into simpler compounds through a metabolic process. TCBS agar, which stands for Thiosulfate Citrate Bile Salt Sucrose agar, is a selective and differential medium used to isolate and identify Vibrio species, including Vibrio cholerae. The agar contains sucrose as a carbohydrate source, and when Vibrio cholerae ferments sucrose, it produces acid, which turns the media yellow. Therefore, TCBS agar is the correct answer because it allows for the identification of Vibrio cholerae based on its ability to ferment sucrose and produce a yellow color on the agar.

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  • 6. 

    DCA is a selective media for identifying enteric bacteria?

    • True

    • False

    Correct Answer
    A. True
    Explanation
    Desoxycholate citrate agar

    Differential, selective media for identifying enteric pathogens.

    Non-lactose fermentors produce colourless colonies and lactose-fermenters produce pink colonies.

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  • 7. 

    Which of the following require chocolate agar to grow?

    • E.coli

    • Neisseria

    • Proteus

    • Salmonella

    Correct Answer
    A. Neisseria
    Explanation
    Neisseria is the correct answer because it is a bacteria that requires chocolate agar to grow. Chocolate agar is a type of nutrient agar that contains lysed red blood cells, which provides essential nutrients for the growth of Neisseria bacteria. This type of agar is commonly used for cultivating fastidious bacteria like Neisseria, as it provides the necessary factors for their growth and allows for better isolation and identification of these bacteria. E. coli, Proteus, and Salmonella do not require chocolate agar for growth, therefore they are not the correct answer.

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  • 8. 

    Bulls eye colonies are formed when what organism grows?

    • Salmonella

    • Yersinia enterocolitica

    • Shigella

    • Staph

    Correct Answer
    A. Yersinia enterocolitica
    Explanation
    Supportive and selective media for the growth of Yersina enterocolitica is yersinia-selective media Appear as red bull's-eye colonies.

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  • 9. 

    GC media is used for the growth of Neisseria?

    • True

    • False

    Correct Answer
    A. True
    Explanation
    GC media, also known as chocolate agar, is a type of culture medium that is commonly used for the growth of Neisseria species. Neisseria is a genus of bacteria that includes important human pathogens such as Neisseria gonorrhoeae and Neisseria meningitidis. These bacteria have specific nutritional requirements, including the need for factors present in blood, which are provided by GC media. Therefore, it is true that GC media is used for the growth of Neisseria.

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  • 10. 

    Bile aesculin agar is used to select for.....

    • Shigella

    • Staph

    • Salmonella

    • Enterococci

    Correct Answer
    A. Enterococci
    Explanation
    Selectively differential agar used to identify enterococcus spp.
    Dark brown/black precipitate forms when organism grows.
    Can also be used to identify klebsiella-serratio-enterobacter group from other enterobactericiae.

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  • 11. 

    Sabouraud Dextrose agar is used to cultivate bacteria.

    • True

    • False

    Correct Answer
    A. False
    Explanation
    Apeptone containing agar

    Used to cultivate fungi

    Has a pH of 5.6 to inhibit bacterial growth

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  • 12. 

    Which of these organisms produce clear zones around them on DNase agar?

    • Staph saprophyticus

    • Moraxella and Staph Aureus

    • Streptococci and staph saprophyticus

    • Streptococci

    Correct Answer
    A. Moraxella and Staph Aureus
    Explanation
    A clear zone around the growth indicates the action of DNAse enzymes (i.e. The organism can use DNA for a source of energy).

    DNAse positive: Moraxella and Staph aureus

    DNAse negative: Coagulase negative staph

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  • 13. 

    Select the lactose fermenting bacteria from the list:

    • Pseudomonas

    • E.coli

    • Shigella

    • Klebsiella

    • Salmonella

    • Proteus

    Correct Answer(s)
    A. E.coli
    A. Klebsiella
    Explanation
    Lactose fermenting bacteria are those that have the ability to break down lactose, a sugar found in milk, into lactic acid. E.coli and Klebsiella are both examples of lactose fermenting bacteria. Pseudomonas, Shigella, Salmonella, and Proteus, on the other hand, are not known for their ability to ferment lactose. Therefore, the correct answer is E.coli and Klebsiella.

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  • Mar 22, 2023
    Quiz Edited by
    ProProfs Editorial Team
  • Apr 21, 2014
    Quiz Created by
    Andrew Blanshard
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