Assessement 2: Wbc Differential And Platelet Estimation

The correct answer is A neutrophil and a monocyte. During a WBC differential count, different types of leukocytes are observed and identified. Neutrophils are a type of white blood cell that are involved in the body's immune response and are responsible for fighting bacterial infections. Monocytes are another type of white blood cell that play a role in the immune response by engulfing and destroying pathogens. Therefore, the presence of a neutrophil and a monocyte indicates a normal immune response in the canine blood sample.

Activated platelets are the best way to describe these cells found during a canine blood film evaluation. Platelets are small, colorless cell fragments that play a crucial role in blood clotting. When platelets become activated, they undergo changes in shape and release various substances that aid in clot formation. In a blood film evaluation, activated platelets may be observed as irregularly shaped cells with granules or other signs of activation. This description accurately identifies the specific type of cells being referred to in the question.

The leukocytes observed during the WBC differential count on canine blood should be classified as lymphocytes.

The leukocytes observed during the WBC differential count of canine blood should be counted as neutrophils. Neutrophils are a type of white blood cell that are involved in the immune response and are responsible for phagocytosis of bacteria and other foreign substances. They have a multi-lobed nucleus and contain granules in their cytoplasm. In the given options, basophils, eosinophils, and lymphocytes are different types of white blood cells, but they have distinct morphological characteristics that differentiate them from neutrophils.

The absolute count for neutrophils can be calculated by multiplying the percentage of neutrophils (36%) with the total WBC count (12,000 per microliter). This gives us 4,320 per microliter.

Basophils are a type of white blood cell that are involved in the immune response and play a role in allergic reactions. They are characterized by their large, dark-staining granules in the cytoplasm. Basophils are relatively rare in the blood, accounting for only a small percentage of white blood cells. In this case, the leukocytes observed in the canine blood sample were classified as basophils based on their characteristic appearance and staining properties.

The estimated number of platelets per microliter of blood is 154,000. This is calculated by taking the average number of platelets counted in each HPF (High Power Field) and multiplying it by the total number of HPFs. In this case, the average number of platelets is (1+13+15+7+0+4+4+0+18+15)/10 = 7.7. Multiplying this by the total number of HPFs (10) gives us 77. Finally, we convert this to platelets per microliter by multiplying by a conversion factor of 2,000 (since there are 2,000 HPFs in a microliter). Therefore, the estimated number of platelets per microliter of blood is 77 x 2,000 = 154,000.

During a WBC differential count of canine blood, these leukocytes should be counted as eosinophils. Eosinophils are a type of white blood cell that play a role in the immune response against parasites and allergies. They are characterized by their granules that stain red with eosin dye. By identifying and counting the eosinophils, it helps in diagnosing and monitoring certain diseases and conditions in the dog's blood.

The technician has determined that the average number of platelets per high power field (HPF) is 18. To estimate the number of platelets per microliter of blood, we need to convert from HPF to microliter. Since the HPF area is known to be 0.1 mm² and the blood smear thickness is approximately 0.1 mm, the volume of blood in the HPF is 0.01 microliters. Dividing the average number of platelets per HPF (18) by the volume of blood in the HPF (0.01 microliters) gives us an estimated number of platelets per microliter of blood, which is 360,000.

The relative count for eosinophils is 15%. This means that out of the total WBC count of 10,000 per microliter, 15% of them are eosinophils.