Flow Cytometry Quiz: Trivia Test!
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In the following quiz, we’re going to be learning all about the technological advancement that allows us to analyze the physical and chemical characteristics of particles in a fluid while it passes through a laser – that’s right, we’re talking about the topic of flow cytometry. What do you know about this process and the reason why we use it? Take the quiz now to let us know! Good luck!
Questions and Answers
- 1.
What do you have to adjust to make sure the emission of fluorescence from a single fluorophore does not bleed into multiple channels?
- A.
Compensation
- B.
Threshold
- C.
Voltage
Correct Answer
A. CompensationExplanation
To ensure that the emission of fluorescence from a single fluorophore does not bleed into multiple channels, compensation needs to be adjusted. Compensation is a technique used in flow cytometry to correct for spectral overlap between fluorochromes. By adjusting the compensation, the emission signals from different fluorophores can be separated and accurately detected in their respective channels, minimizing any cross-contamination or bleed-through.Rate this question:
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- 2.
What does light emit as forward side scatter (FSC) measure?
- A.
Cell size
- B.
Cell granularity / complexity
- C.
Cell surface marker fluorescence
Correct Answer
A. Cell sizeExplanation
Forward side scatter (FSC) is a measure of the size of cells. When light passes through a cell, larger cells scatter more light in the forward direction compared to smaller cells. Therefore, FSC can be used as an indicator of cell size. This measurement is commonly used in flow cytometry to assess the physical characteristics of cells and distinguish between different cell populations based on their size.Rate this question:
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- 3.
What are the 2 lasers included in the 4 colors BD FACSCalibur?
- A.
An argon 488 nm laser and a red 635 nm diode laser
- B.
An argon red 488 nm laser and a blue 635 nm diode laser
- C.
A blue diode 488 nm laser and a red 635 nm argon laser
Correct Answer
A. An argon 488 nm laser and a red 635 nm diode laserExplanation
The correct answer is "An argon 488 nm laser and a red 635 nm diode laser." This answer accurately identifies the two lasers included in the 4 colors BD FACSCalibur. The argon laser emits light at a wavelength of 488 nm, while the red diode laser emits light at a wavelength of 635 nm. These two lasers provide different colors of light that can be used in flow cytometry analysis.Rate this question:
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- 4.
The FL4 channel measures emission in the far red range. What fluorophores does channel 4 (FL4) measure? FL4 - far-red emission (filter: 661/12 nm)
- A.
FITC, Alexa488, CFSE
- B.
Allophycocyanin (APC)
- C.
PerCP
Correct Answer
B. AllopHycocyanin (APC)Explanation
The FL4 channel measures emission in the far red range, specifically at a wavelength of 661/12 nm. Allophycocyanin (APC) is a fluorophore that emits in the far red range, making it the appropriate answer for FL4. FITC, Alexa488, and CFSE emit in different ranges and therefore would not be measured by FL4. PerCP also emits in the far red range, but it is not listed as an option, so it is not the correct answer.Rate this question:
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- 5.
The FL1 channel measures emission in the green range. What fluorophores does channel 1 (FL1) measure? FL1 - green emission (filter: 530/30 nm)
- A.
FITC, Alexa488, CFSE
- B.
Phycoerythrin (PE)
- C.
Allophycocyanin (APC)
Correct Answer
A. FITC, Alexa488, CFSEExplanation
FL1 channel measures emission in the green range, specifically using a filter with a wavelength of 530/30 nm. The fluorophores FITC, Alexa488, and CFSE all emit in the green range, making them detectable by the FL1 channel. Phycoerythrin (PE) and Allophycocyanin (APC) do not emit in the green range and therefore would not be measured by FL1.Rate this question:
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- 6.
The FL3 channel measures emission in the red range. What fluorophores does channel 3 (FL3) measure? FL3 - red emission (filter: 670 nm LP)
- A.
Allophycocyanin (APC)
- B.
PerCP
- C.
FITC, Alexa488, CFSE
Correct Answer
B. PerCPExplanation
FL3 channel measures emission in the red range, specifically with a filter at 670 nm LP. Among the given options, PerCP is the only fluorophore that emits in the red range, making it the correct answer. Allophycocyanin (APC) emits in the far-red range, FITC, Alexa488, and CFSE emit in the green range, so they are not measured by FL3 channel.Rate this question:
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- 7.
The FL2 channel measures emission in the red-orange range. What fluorophores does channel 2 (FL2) measure? FL2 - red-orange emission (filter: 585/42 nm)
- A.
FITC, Alexa488, CFSE
- B.
Phycoerythrin (PE)
- C.
PerCP
Correct Answer
B. pHycoerythrin (PE)Explanation
FL2 channel measures emission in the red-orange range, specifically at a wavelength of 585/42 nm. Phycoerythrin (PE) is a fluorophore that emits in this range, making it the correct answer. FITC, Alexa488, and CFSE do not emit in the red-orange range, so they are not measured by FL2. PerCP also does not emit in the red-orange range, so it is not measured by FL2 either.Rate this question:
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- 8.
What does light emit as a side scatter (SSC) measure?
- A.
Cell size
- B.
Cell granularity / complexity
- C.
Cell surface marker fluorescence
Correct Answer
B. Cell granularity / complexityExplanation
Side scatter (SSC) is a measurement used in flow cytometry to assess the granularity or complexity of cells. When light passes through a cell, it scatters in different directions depending on the size and internal complexity of the cell. Cells with more internal structures or granules will scatter more light, resulting in a higher SSC signal. Therefore, the correct answer is that light emitted as a side scatter (SSC) measure reflects the cell's granularity or complexity.Rate this question:
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- 9.
Which part of the cytometer brings the cells to the interrogation point where the cells meet the laser?
- A.
Electronics
- B.
Fluidics
- C.
Optics
Correct Answer
B. FluidicsExplanation
The correct answer is Fluidics. In a cytometer, the fluidics system is responsible for bringing the cells to the interrogation point where they meet the laser. The fluidics system controls the flow of the sample containing the cells and ensures that the cells are properly aligned with the laser beam for analysis. It helps in maintaining a steady flow of cells and prevents clogging or blockages that could affect the accuracy of the measurements.Rate this question:
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- 10.
Which part of the cytometer consists of the excitation sources and detectors?
- A.
Optics
- B.
Electronics
- C.
Fluidics
Correct Answer
A. OpticsExplanation
The correct answer is Optics. In a cytometer, the optics section is responsible for providing the excitation sources, such as lasers or lamps, which emit light at specific wavelengths. It also includes detectors, such as photomultiplier tubes or avalanche photodiodes, which capture the emitted light and convert it into electrical signals. The optics section plays a crucial role in illuminating the sample and detecting the signals generated by the cells or particles being analyzed.Rate this question:
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- 11.
Which part of the cytometer converts the light signal to voltage so it can be interpreted through computer software?
- A.
Fluidics
- B.
Optics
- C.
Electronics
Correct Answer
C. ElectronicsExplanation
The electronics of a cytometer convert the light signal detected by the optics into voltage signals that can be interpreted by computer software. This conversion allows for the analysis and interpretation of the data collected by the cytometer. Without the electronics, the light signals would not be able to be processed and analyzed by the computer software.Rate this question:
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- 12.
To which filters those definitions correspond a) transmit light in the range of specified wavelength, b) transmit light equal to or longer than the specified wavelength, and c) transmit light equal to or shorter than the specified wavelength?
- A.
Shortpass, bandpass, longpass
- B.
Longpass, shortpass, bandpass
- C.
Bandpass, longpass, shortpass
Correct Answer
C. Bandpass, longpass, shortpassExplanation
The correct answer is Bandpass, longpass, shortpass. Bandpass filters allow only a specific range of wavelengths to pass through, transmitting light in the range of the specified wavelength. Longpass filters transmit light equal to or longer than the specified wavelength, allowing longer wavelengths to pass through. Shortpass filters transmit light equal to or shorter than the specified wavelength, allowing shorter wavelengths to pass through. Therefore, the given answer aligns with the definitions provided.Rate this question:
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- 13.
How do you choose a fluorophore?
- A.
By its excitation and emission wavelengths
- B.
By the laser and detectors/filters present in the cytometer
- C.
All answers
Correct Answer
C. All answersExplanation
The correct answer is "All answers". When choosing a fluorophore, both the excitation and emission wavelengths need to be considered. The excitation wavelength should match the laser used for excitation, and the emission wavelength should be compatible with the detectors and filters present in the cytometer. Therefore, both factors mentioned in the options are important in selecting a fluorophore.Rate this question:
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Current Version
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Mar 22, 2023Quiz Edited by
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Apr 21, 2011Quiz Created by
ETP_MNI
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