Sterilization Questions: Microbiology Quiz!

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  • 1/10 Questions

    The initial microbial contamination level influences the time-dependent process( elimination of viable microorganisms)

    • True
    • False
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About This Quiz

Explore the Sterilization Questions: Microbiology Quiz to test your knowledge of microbial contamination, sterilization methods, and sterilizer design. This quiz assesses understanding of crucial sterilization processes, vital for professionals in microbiology and related fields.

Sterilization Questions: Microbiology Quiz! - Quiz

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  • 2. 

    Ethylene oxide gas is highly explosive in mixtures of >3.6% v/v in air.

    • True

    • False

    Correct Answer
    A. True
    Explanation
    Ethylene oxide gas is highly explosive in mixtures of >3.6% v/v in air. This means that if the concentration of ethylene oxide gas in the air is higher than 3.6% by volume, it can easily ignite and explode. This highlights the extreme danger associated with ethylene oxide gas and emphasizes the need for proper handling and storage measures to prevent accidents and ensure safety.

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  • 3. 

    The elevated temperatures generally associated with moist heat sterilization methods can only be achieved by:

    • Pure ethylene oxide gas

    • S broad-spectrum biocidal activity

    • Oxidative processes

    • Generation of steam under pressure

    Correct Answer
    A. Generation of steam under pressure
    Explanation
    The correct answer is the generation of steam under pressure. Moist heat sterilization methods, such as autoclaving, rely on the generation of steam under pressure to achieve elevated temperatures. This process effectively kills microorganisms by denaturing proteins and disrupting cell membranes. Pure ethylene oxide gas is used in another type of sterilization method called gas sterilization. While ethylene oxide gas also has broad-spectrum biocidal activity, it does not rely on elevated temperatures. Oxidative processes can be involved in sterilization methods, but they are not specific to moist heat sterilization.

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  • 4. 

    Which of the following is INCORRECT:

    • Trapping within the matrix of the filter material is one of the mechanisms of filtration

    • The principal application of sterilizinggrade filters related to the  venting systems on fermenters

    • Filtration sterilization cannot be used for the clarification and sterilization of liquids and gases.

    •  UV light used  in the sterilization of the treatment of manufacturing-grade water.

    Correct Answer
    A. Filtration sterilization cannot be used for the clarification and sterilization of liquids and gases.
    Explanation
    Filtration sterilization can indeed be used for the clarification and sterilization of liquids and gases. This method involves passing the liquid or gas through a filter that traps and removes microorganisms, thereby clarifying and sterilizing the substance. Therefore, the statement that filtration sterilization cannot be used for clarification and sterilization of liquids and gases is incorrect.

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  • 5. 

    Which of the following is INCORRECT:

    • Pure ethylene oxide gas can be used below atmospheric pressure in sterilizer chambers from which all air has been removed

    • Formaldehyde  limits the packaging materials that can be employed to principally paper and cotton fabric.

    •  Formaldehyde cannot normally be detected by smell at concentrations lower than those permitted in the atmosphere

    • Ethylene chlorohydrin are potentially mutagenic and carcinogenic  

    Correct Answer
    A.  Formaldehyde cannot normally be detected by smell at concentrations lower than those permitted in the atmosphere
    Explanation
    Formaldehyde can be detected by smell at concentrations lower than those permitted in the atmosphere.

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  • 6. 

    Ethylene oxide sterilization needs a higher temperature than formaldehyde.

    • True

    • False

    Correct Answer
    A. False
    Explanation
    Ethylene oxide sterilization does not necessarily require a higher temperature than formaldehyde. The temperature required for sterilization depends on various factors such as the type and size of the item being sterilized, the desired level of sterilization, and the specific sterilization method being used. While ethylene oxide sterilization typically involves lower temperatures, formaldehyde can also be used at lower temperatures for certain applications. Therefore, the statement that ethylene oxide sterilization requires a higher temperature than formaldehyde is false.

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  • 7. 

    Which of the following is CORRECT regarding Sterilizer design and  Sterilizer operation.

    • Shelves within the chamber are perforated to prevent cool spots developing inside the chamber. 

    • A fixed temperature sensor connected to a chart recorder provides a permanent record of the sterilization cycle.

    • Appropriate door-locking controls should be incorporated to  shorten the heating-up stage

    • A fan is fitted to the rear of the oven to prevent interruption of a sterilization cycle once begun

    Correct Answer
    A. A fixed temperature sensor connected to a chart recorder provides a permanent record of the sterilization cycle.
  • 8. 

    Which of the following is CORRECT:

    • Porous loads (i.e. dressings) are of the types of  ‘portable’ sterilizers

    •  large-scale sterilizers generally have internal electric heaters

    • Bottled fluid sterilizers employing a minimum temperature of 121°C.

    • ‘portable’ sterilizers, for routine hospital or industrial use

    Correct Answer
    A. Bottled fluid sterilizers employing a minimum temperature of 121°C.
  • 9. 

    Which of the following is CORRECT:

    • Radiation sterilization is generally applied to articles in the aqueous state

    • Radiation sterilization has not proved to be a method of industrial sterilization of heat-sensitive products.

    • In the case of UV light , mould spores prove to be most resistant

    • Electron or gamma-irradiation is not as efficient a sterilization method as UV irradiation

    Correct Answer
    A. In the case of UV light , mould spores prove to be most resistant
    Explanation
    Mould spores are known to be highly resistant to UV light, making them the most resistant when it comes to sterilization using UV light. This implies that other microorganisms may not be as resistant and can be effectively sterilized using UV light.

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  • 10. 

    Dry heat sterilization used for hydrogenation of glassware.

    • True

    • False

    Correct Answer
    A. False
    Explanation
    Dry heat sterilization is a method used to sterilize glassware by exposing it to high temperatures in the absence of moisture. It is not used for hydrogenation, which is a chemical reaction that involves the addition of hydrogen to a compound. Therefore, the statement is false.

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Quiz Review Timeline (Updated): Mar 22, 2023 +

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  • Current Version
  • Mar 22, 2023
    Quiz Edited by
    ProProfs Editorial Team
  • Aug 16, 2020
    Quiz Created by
    Pharmacgy
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