1.
Blastomyces dermatitidis at 35-37 degrees celsius on BHI, s yeastlike cells are seen; these cells have a bud on a broad base and appear to be thick walled and double contoured; the bud often remains attached until it becomes the same size as the mother cell giving them an _______ appearance.
Correct Answer
B. Hourglass
Explanation
The correct answer is "hourglass" because the description provided suggests that the yeastlike cells of Blastomyces dermatitidis have a bud on a broad base and a thick-walled, double-contoured appearance. The bud remains attached until it becomes the same size as the mother cell, creating an hourglass shape.
2.
Identification of Blastomyces dermatitidis involves the conversion of the mold to the yeast form in order to confirm the identification.
Correct Answer
A. True
Explanation
The identification of Blastomyces dermatitidis requires converting the mold form of the organism into the yeast form. This conversion is necessary to confirm the identification of the fungus. Therefore, the statement "Identification of Blastomyces dermatitidis involves the conversion of the mold to the yeast form in order to confirm the identification" is true.
3.
Paracoccidioides brasiliensis often occurs in adult _______; this may be due to their hormonal makeup and their occupation.
Correct Answer
B. Male
Explanation
Paracoccidioides brasiliensis often occurs in adult males; this may be due to their hormonal makeup and their occupation.
4.
Mycelial forms of Paracoccidiodes brasiliensis mature within ______ days.
Correct Answer
C. 21
Explanation
The mycelial forms of Paracoccidiodes brasiliensis take 21 days to mature.
5.
At 35-37 degrees Celsius on BHI, large, round, fairly thick-walled cells with single and multiple buds. Paracoccidiodes brasiliensis buds are attached to the mother cell by narrow connections and may almost completely surround the cell, giving the characteristic "______ " or "_______ " appearance.
Correct Answer
A. Ship wheel or mariner's wheel
Explanation
Paracoccidioides brasiliensis buds are attached to the mother cell by narrow connections and may almost completely surround the cell, giving the characteristic "ship wheel" or "mariner's wheel" appearance. This description suggests that the arrangement of the buds resembles the spokes of a ship wheel or mariner's wheel, with the mother cell at the center and the buds radiating outwards like the spokes.
6.
Penicillum marneffei is endemic to areas of ______________
Correct Answer
D. Southeast Asia
Explanation
Penicillium marneffei is endemic to Southeast Asia. This means that the fungus is naturally found and restricted to this specific region. It is not found in Africa, South America, or North America. The term "endemic" indicates that the organism is native to and restricted to a particular geographic area, in this case, Southeast Asia.
7.
Colonies of Penicillium marneffei becoming mature in ______ days at 25-30 degrees; yeastlike forms develop more slowly at 35-37 degrees celsius.
Correct Answer
C. 3
Explanation
The colonies of Penicillium marneffei become mature in 3 days at 25-30 degrees Celsius. The yeastlike forms, however, develop more slowly at 35-37 degrees Celsius.
8.
Penicillium marneffei is not inhibited by cycloheximide
Correct Answer
B. False
Explanation
Penicillium marneffei is inhibited by cycloheximide. Cycloheximide is an antifungal agent that works by inhibiting protein synthesis in fungi. Since Penicillium marneffei is a fungus, it would be affected by cycloheximide and its growth would be inhibited. Therefore, the correct answer is False.
9.
Conversion of Penicillium marneffei from the mold to the yeast form may take up to _______ days; most rapidly accomplish by using BHI broth on a shaker.
Correct Answer
B. 14
Explanation
The conversion of Penicillium marneffei from the mold to the yeast form may take up to 14 days. This process is most rapidly accomplished by using BHI broth on a shaker.
10.
Penicillium marneffei has smooth conidiophores with _____ terminal metulae, each metula bearing 4-6 phialides.
Correct Answer
B. 4-5
Explanation
Penicillium marneffei has smooth conidiophores with 4-5 terminal metulae, each metula bearing 4-6 phialides. This means that each conidiophore of Penicillium marneffei has 4-5 branches at the tip, and each branch has 4-6 phialides. The number of terminal metulae and phialides is important for the identification and classification of fungi.
11.
Are buds produced when dealing with Penicillium marneffei?
Correct Answer
B. No
Explanation
Buds are not produced when dealing with Penicillium marneffei. Penicillium marneffei is a dimorphic fungus that reproduces by forming spores called conidia, rather than buds. Buds are typically produced by yeast cells during a process called budding, where a small daughter cell forms and eventually separates from the mother cell. However, this process does not occur in Penicillium marneffei.