Cytology In Clinical Practice
This presentation given by Tracy Stokol, BVSc, PhD and Associate Professor in Clinical Pathology at Cornell University, consists of a review of sample collection, smear preparation and staining of cytology samples. The lecture will conclude with instructions on general slide evaluation for common lesions diagnosed on cytology and clinical cases to practice your skills.
Date: July 2014
Venue: 2014 ASPCA/Cornell Maddie's® Shelter Medicine Conference
- 1.
We hope you have enjoyed the presentation. If you receive a passing score, a Certificate of Attendance in your name will be awarded at the end of this quiz. Please let us know if you have viewed the entire presentation.
- A.
I certify that I have viewed the entire presentation.
- B.
I did not view the entire presentation.
Correct Answer
A. I certify that I have viewed the entire presentation. -
- 2.
Which of the following is true about cytology samples?
- A.
The smear type is not dictated by the type of sample (e.g., fluid, solid tissue aspirate, biopsy).
- B.
History details are not required.
- C.
They should be keep out of contact with formalin.
- D.
Unstained slides are coverslipped.
- E.
All of the above.
Correct Answer
C. They should be keep out of contact with formalin. -
- 3.
For the collection of body cavity fluids, you should refrigerate tubes and slides until submission.
- A.
True
- B.
False
Correct Answer
B. False -
- 4.
Which of the following is not true regarding a “squash smear”?
- A.
You should press the bottom and top slide together.
- B.
Slides must be at a 90 degree angle.
- C.
You should put your drop of blood or aspirate close to the frosted edge.
- D.
A and B.
- E.
A, B and C.
Correct Answer
D. A and B. -
- 5.
The type of slide made from fluid:
- A.
Is dictated by the clarity of the sample.
- B.
Is dictated by the color of the sample.
- C.
Is dictated by the amount of the sample.
- D.
Is dictated by the weight of the sample.
- E.
All of the above.
Correct Answer
A. Is dictated by the clarity of the sample. -
- 6.
When making slides of solid tissue:
- A.
Use fresh cut surface to avoid ulcerations.
- B.
Blot first for impression smears.
- C.
Scalpel blade scrapings are hard to stain adequately.
- D.
All of the above.
- E.
A and B.
Correct Answer
D. All of the above. -
- 7.
Consistency is key with cytology (i.e., develop a system for slide examination, be thorough and consistent, use low power then high power).
- A.
True
- B.
False
Correct Answer
A. True -
- 8.
Use low power (10x) of the microscope to:
- A.
Identify good areas to look at (i.e., cells are well spread and well stained).
- B.
Initially identify anything that appears strange.
- C.
Take a closer look at unusual areas on the slide.
- D.
Visualize cells with oil immersion.
- E.
A and B.
Correct Answer
E. A and B. -
- 9.
Use high power (40x) of the microscope to:
- A.
Further evaluate areas that appear strange.
- B.
Visualize cells with oil immersion.
- C.
Identify blood, inflammation and tissue cells.
- D.
All of the above.
- E.
None of the above.
Correct Answer
D. All of the above. -
- 10.
Old samples that are used for cytology can produce:
- A.
Erythrocytosis.
- B.
Bacteriopsonin.
- C.
Cell lysis.
- D.
Anti-mortem: clostridial overgrowth, exfoliation of mesothelial cells, autolysis.
- E.
All of the above.
Correct Answer
C. Cell lysis. -
- 11.
What factors can negatively impact the accuracy of cytology findings?
- A.
Inadequate staining (insufficient time, smear too thick)
- B.
Time for the stained sample to air dry
- C.
Diff-quick staining
- D.
Impression smears of fresh cut samples
- E.
All of the above
Correct Answer
A. Inadequate staining (insufficient time, smear too thick) -
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