DNA Extraction Warm Up 1

Isopropyl alcohol is added to the wheat germ, distilled water, detergent, and sodium chloride solution. This chemical is commonly used as a solvent and disinfectant. It is likely added to the solution to sterilize the equipment or to extract certain compounds from the mixture. Isopropyl alcohol has a low boiling point, which makes it easy to evaporate and leave behind the desired components.

Isopropyl alcohol should be added to the mixture of wheat germ, distilled water, detergent, and sodium chloride solution. The layer of isopropyl alcohol should be on the top of the column in the test tube.

Strawberries typically have 8 sets of chromosomes in their nucleus. This means that they are octoploid, with each set of chromosomes consisting of 8 chromosomes. This is in contrast to humans, who are diploid and have 2 sets of chromosomes. The number of sets of chromosomes in an organism's nucleus determines its ploidy level, which can vary among different species.

The correct answer is detergent solution. Detergent solution is added to the wheat germ and distilled water in order to break down the cell membranes and release the DNA. Detergents are able to disrupt the lipid bilayer of the cell membrane, allowing the DNA to be extracted.

In this experiment, various chemicals are added to the test tube, including wheat germ, distilled water, detergent solution, and sodium chloride solution. The question asks which layer should be in the bottom of the column in the test tube. Since the question does not specify any specific layers or column, it is difficult to determine the exact answer. However, based on the given options, it can be inferred that the wheat germ is the correct answer because it is the only option that is not a solution or a chemical, but rather a solid substance.

After preparing the test tube, distilled water is added to the wheat germ. Distilled water is used because it is free from impurities and contaminants. It serves as a control in the experiment, allowing for accurate comparison and measurement of the effects of other substances added to the wheat germ.

Wheat (Triticum aestivum) has 42 diploid chromosomes in its nucleus during the resting stage. Diploid refers to the number of chromosomes in a cell, where each chromosome is present in pairs. In the case of wheat, there are 21 pairs of chromosomes, resulting in a total of 42 chromosomes.

The isopropyl alcohol is described as "extremely cold" when it is mixed into the test tube. This suggests that the temperature of the isopropyl alcohol is significantly lower than room temperature, warm, hot, or cold. The term "extremely cold" implies that the isopropyl alcohol is at a very low temperature, possibly close to or below freezing point.

The correct answer is "Extracted DNA" because the question is asking for the chemical that is added to the mixture of wheat germ, distilled water, detergent, and sodium chloride solution. The extracted DNA is the chemical that is added to the mixture to observe its effects or analyze it further. The other options mentioned are not chemicals that are added to the mixture but rather components or solutions that are already present in the experiment.

The chemicals that should be mixed into the "wheat germ" layer are distilled water, sodium chloride solution, and detergent solution. These chemicals are commonly used in DNA extraction protocols. Distilled water is used as a solvent to dissolve other substances. Sodium chloride solution is used to create a hypertonic environment, which helps to separate DNA from other cellular components. Detergent solution is used to break down the cell membranes and release the DNA. Therefore, mixing these chemicals into the "wheat germ" layer would facilitate the extraction of DNA from wheat germ.