Molecular Biology Quiz: DNA Transcription, Translation, Replication
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Test your knowledge with this molecular biology quiz. Molecular Biology is known as the field of biology that studies the composition, structure as well as interactions of cellular molecules. Transcription is the first step of gene expression, where the messenger RNA is decoded in a ribosome to produce polypeptide, which later folds into an active protein and performs its functions in the cell. We tried to understand the structure, function, and processes of DNA and RNA in the cell. See how much you understand about it by taking this quiz.
DNA Transcription, Translation, Replication Questions and Answers
1.
DNA and RNA are different because:
A.
DNA is only found in the nucleus, while RNA can travel anywhere in the cell.
B.
DNA is double-stranded, while RNA is triple-stranded.
C.
DNA uses Uracil while RNA uses Thymine.
D.
DNA is made of four bases, while RNA is made of five bases.
E.
None of the above
Correct Answer
E. None of the above
Explanation DNA and RNA are different nucleic acids with distinct structures and functions, but the options provided do not accurately capture the differences between them. DNA can be found in both the nucleus and the mitochondria, RNA is typically single-stranded, RNA uses uracil while DNA uses thymine, and both DNA and RNA are made of four bases (adenine, guanine, cytosine, and either thymine in DNA or uracil in RNA).
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2.
Using the following DNA sequence, what would be the corresponding DNA strand?
ATTGCGACCGAG
A.
UAACGCUGGCUC
B.
AUUGCGACCGAG
C.
TAACGCTGGCTC
D.
UATCGCTGGCUC
E.
None of the above are correct.
Correct Answer
C. TAACGCTGGCTC
Explanation To find the corresponding DNA strand, you can use the base-pairing rules in DNA, which specify how the individual nucleotides in one strand pair with those in the other strand. These rules are as follows:
- Adenine (A) pairs with Thymine (T).
- Thymine (T) pairs with Adenine (A).
- Cytosine (C) pairs with Guanine (G).
- Guanine (G) pairs with Cytosine (C).
In the given DNA sequence "ATTGCGACCGAG," you can find the corresponding DNA strand by applying these base-pairing rules:
- A (Adenine) in the original sequence pairs with T (Thymine) in the complementary strand.
- T (Thymine) in the original sequence pairs with A (Adenine) in the complementary strand.
- C (Cytosine) in the original sequence pairs with G (Guanine) in the complementary strand.
- G (Guanine) in the original sequence pairs with C (Cytosine) in the complementary strand.
Following these rules, you can create the complementary DNA strand by replacing each nucleotide in the original sequence with its complementary base. This results in the complementary DNA strand "TAACGCTGGCTC."
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3.
Which of the following is NOT a type of RNA?
A.
Messenger
B.
Transcription
C.
Transfer
D.
Ribosomal
E.
These are all types of RNA
Correct Answer
B. Transcription
Explanation Transcription is not a type of RNA. Transcription is a process in which DNA is converted into RNA, specifically messenger RNA (mRNA). Messenger RNA, transfer RNA (tRNA), and ribosomal RNA (rRNA) are all types of RNA that play different roles in protein synthesis. Therefore, the correct answer is transcription as it is a process, not a type of RNA.
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4.
Where does DNA replication occur?
A.
In the nucleus
B.
In the cytoplasm
C.
At the ribosome
D.
In the mitochondrion
Correct Answer
A. In the nucleus
Explanation DNA replication occurs in the nucleus of a cell. The nucleus contains the genetic material, which is stored in the form of DNA. During DNA replication, the double-stranded DNA molecule unwinds, and each strand serves as a template for the synthesis of a new complementary strand. This process occurs in the nucleus because that is where the DNA is located and where the necessary enzymes and proteins for replication are present. The cytoplasm is where protein synthesis occurs at the ribosomes, and the mitochondrion is responsible for energy production, not DNA replication.
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5.
When transcription occurs, what molecules are involved?
A.
DNA and rRNA
B.
DNA, mRNA, amino acids, and rRNA
C.
DNA and tRNA
D.
DNA and mRNA
Correct Answer
D. DNA and mRNA
Explanation During transcription, DNA is used as a template to synthesize mRNA molecules. This process involves the enzyme RNA polymerase, which binds to the DNA strand and reads the nucleotide sequence. As the RNA polymerase moves along the DNA, it synthesizes a complementary mRNA molecule by adding nucleotides according to the DNA template. Therefore, the correct answer is DNA and mRNA, as these are the molecules directly involved in the transcription process.
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6.
When translation occurs, what molecules are involved?
A.
DNA, mRNA, tRNA, rRNA, and amino acids
B.
DNA and mRNA
C.
DNA and rRNA
D.
DNA, rRNA, and tRNA
Correct Answer
A. DNA, mRNA, tRNA, rRNA, and amino acids
Explanation This option correctly identifies all the components critical to the translation process. However, it is important to note that DNA itself is not directly involved in the translation process; rather, its information is transcribed to mRNA, which then participates in translation. So the presence of "DNA" in this list might be considered incorrect depending on the context or the strictness of the interpretation. If considering only the direct participants of the translation machinery itself, the correct components are mRNA, tRNA, rRNA, and amino acids.
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7.
What would be the corresponding sequence to the following DNA sequence as a result of TRANSCRIPTION?
TAAGCATTCCGAG
A.
ATTCGTAAGGCTC
B.
AUUCGUAAGGCUC
C.
UTTCGTUUGGCTC
D.
UUUGCUUUCCGUG
Correct Answer
B. AUUCGUAAGGCUC
Explanation During transcription, DNA is converted into RNA. The corresponding RNA sequence is formed by replacing each DNA base with its RNA complement: A with U, T with A, C with G, and G with C. Therefore, the corresponding RNA sequence to the given DNA sequence "TAAGCATTCCGAG" would be "AUUCGUAAGGCUC".
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8.
Where in the cell would rRNA be found?
A.
Nucleus
B.
Ribosomes
C.
Golgi Apparatus
D.
Chloroplast
Correct Answer
B. Ribosomes
Explanation rRNA, or ribosomal RNA, is a type of RNA molecule that is a key component of ribosomes. Ribosomes are cellular structures responsible for protein synthesis. They consist of a large and small subunit, both of which contain rRNA. Therefore, rRNA would be found in ribosomes, making the correct answer "Ribosomes".
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9.
What is the role of messenger RNA?
A.
To bring the DNA message to the mitochondrion.
B.
To bring the tRNA message to the nucleus.
C.
To bring the DNA message to the ribosome.
D.
To take amino acids to the ribosome.
Correct Answer
C. To bring the DNA message to the ribosome.
Explanation Messenger RNA (mRNA) plays a crucial role in protein synthesis. It carries the genetic information from DNA in the nucleus to the ribosomes in the cytoplasm. The DNA message is transcribed into mRNA, which then serves as a template for protein synthesis. The ribosomes read the mRNA and assemble amino acids in the correct order to form a protein. Therefore, the correct answer is that the role of messenger RNA is to bring the DNA message to the ribosome.
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10.
An mRNA codon has the following sequence: ACU
What is the corresponding tRNA sequence called, and what would the sequence be?
A.
Codex; UGA
B.
Codon; TGA
C.
TCode; TGU
D.
Anticodon; UGA
Correct Answer
D. Anticodon; UGA
Explanation The given mRNA codon sequence is ACU. The corresponding tRNA sequence is called an anticodon. The anticodon sequence for ACU is UGA.
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