1.
When using a compound microscope, objective lenses can be found to have a magnification of all of the following, EXCEPT?
Correct Answer
E. 1000X
Explanation
A compound microscope has two lenses: an eyepiece lens and objective lens. The eyepiece lens usually has a magnification of 10X. There are objective lenses on the revolving nosepiece with varying magnifications. Most compound microscopes have objective lenses with magnification of 4X, 10X, and 40X. Some compound microscopes also have an oil immersion lens with a magnification of 100X.
2.
What is "compound microscope"?
Correct Answer
D. Microscope with two lenses
Explanation
A compound microscope has two lenses: an eyepiece lens and objective lens. The eyepiece lens usually has a magnification of 10X. There are objective lenses on the revolving nosepiece with varying magnifications. Most compound microscopes have objective lenses with magnification of 4X, 10X, and 40X. Some compound microscopes also have an oil immersion lens with a magnification of 100X.
3.
What is the total magnification achieved with a compound microscope?
Correct Answer
D. Magnification of ocular lens multiplied by the magnification of the objective
Explanation
To calculate the total magnification achieved with a compound microscope, the magnification of the ocular lens is multiplied by the magnification of the objective lens. For example, if viewing a sample with the 40x objective, the total magnification would be calculated as follows: a 10X ocular lens used with a 40X objective lens, the total magnification is 400X (10 x 40).
4.
What is the maximum resolving power seen with a compound microscope?
Correct Answer
B. 2 micrometers
Explanation
Resolving power is the ability to see two things as discrete images. With normal vision, there is a resolving power of about of 100 micrometers. A compound microscope has a resolving power of approximately .2 micrometers. In other words, two marks .2 micrometers apart can be seen as two distinct entities. Any closer than this, they are perceived as one object.
5.
What is the turret?
Correct Answer
B. Nosepiece
Explanation
The base is the bottom of the microscope. The revolving nosepiece is also called a turret. The objective lens are attached to the nosepiece (or turret). The slide rests on the stage. The tube is the structure which connects the eyepiece to the objective lenses (it is shaped like a tube; thus, its name). The diaphragm controls the diameter of the cone of light.
6.
On a microscope, what structure connects the eyepiece to the objective lens?
Correct Answer
D. Tube
Explanation
The base is the bottom of the microscope. The revolving nosepiece is also called a turret. The objective lens are attached to the nosepiece (or turret). The slide rests on the stage. The tube is the structure which connects the eyepiece to the objective lenses (it is shaped like a tube; thus, its name). The diaphragm controls the diameter of the cone of light.
7.
In a good compound microscope, the focus knob does not have to be readjusted
when changing the magnification. What is this phenomenon called?
Correct Answer
A. Parfocal
Explanation
Parafocal is the term used for a microscope if the focus knob does not have to be readjusted when changing the magnifications. This phenomenon is seen with good compound microscopes. In other words, when the specimen is in focus at 4X and the objective is switched to 10X, the specimen remains in focus.
8.
What is another name for the light microscope?
Correct Answer
B. Compound microscope
Explanation
A simple microscope has only one lens. A compound microscope utilizes two lenses: an ocular lens and an objective lens. The compound microscope is also referred to as a "light microscope" or "bright field microscope". A phase contrast microscope is useful for examining living cells, because the specimen does not need to be stained. A dissection microscope uses low power magnification. Things appear three dimensional with a dissection microscope. A transmission electron microscope does not use light, but rather a beam of electrons
9.
Which microscope does not rely on visible light?
Correct Answer
E. Transmission electron microscope
Explanation
A simple microscope has only one lens. A compound microscope utilizes two lenses: an ocular lens and an objective lens. The compound microscope is also referred to as a "light microscope" or "bright field microscope". A phase contrast microscope is useful for examining living cells, because the specimen does not need to be stained. A dissection microscope uses low power magnification. Things appear three dimensional with a dissection microscope. A transmission electron microscope does not use light, but rather a beam of electrons.
10.
Which microscope makes things appear three dimensional?
Correct Answer
D. Dissection microscope
Explanation
A simple microscope has only one lens. A compound microscope utilizes two lenses: an ocular lens and an objective lens. The compound microscope is also referred to as a "light microscope" or "bright field microscope". A phase contrast microscope is useful for examining living cells, because the specimen does not need to be stained. A dissection microscope uses low power magnification. Things appear three dimensional with a dissection microscope. A transmission electron microscope does not use light, but rather a beam of electrons.
11.
When using a compound microscope, what is the magnification of the oil immersion lens?
Correct Answer
D. 100X
Explanation
A compound microscope has two lenses: an eyepiece lens and objective lens. The eyepiece lens usually has a magnification of 10X. There are objective lenses on the revolving nosepiece with varying magnifications. Most compound microscopes have objective lenses with magnification of 4X, 10X, and 40X. Some compound microscopes also have an oil immersion lens with a magnification of 100X.
12.
What is the usual magnification of the ocular lens on a compound microscope?
Correct Answer
B. 10X
Explanation
The usual magnification of an ocular lens on a compound microscope is 10X. Some microscopes have a 15X eyepiece lens. The ocular lens is the lens at the top of the tube, the one that you first look through when using a microscope. It is also called the eyepiece lens.
13.
When using oil immersion to view a tissue, what is the refractive index of the oil?
Correct Answer
C. Same as glass
Explanation
With light microscopy, there normally is a space of air between the slide and the lens. Oil immersion replaces that space of air with oil. The refractive index of the oil is the same as glass
14.
What is the role of the condenser lens?
Correct Answer
C. Focus the light on the specimen
Explanation
The role of the condenser lens is to focus light on the specimen. It is used with higher magnifications.
15.
On a microscope, what structure varies the diameter of the cone of light?
Correct Answer
E. DiapHragm
Explanation
The base is the bottom of the microscope. The revolving nosepiece is also called a turret. The objective lens are attached to the nosepiece (or turret). The slide rests on the stage. The tube is the structure which connects the eyepiece to the objective lenses (it is shaped like a tube; thus, its name). The diaphragm controls the diameter of the cone of light.
16.
Where do you place the slide when using a microscope?
Correct Answer
C. Stage
Explanation
The base is the bottom of the microscope. The revolving nosepiece is also called a turret. The objective lens are attached to the nosepiece (or turret). The slide rests on the stage. The tube is the structure which connects the eyepiece to the objective lenses (it is shaped like a tube; thus, its name). The diaphragm controls the diameter of the cone of light.
17.
What is the bottom of a microscope called?
Correct Answer
A. Base
Explanation
The base is the bottom of the microscope. The revolving nosepiece is also called a turret. The objective lens are attached to the nosepiece (or turret). The slide rests on the stage. The tube is the structure which connects the eyepiece to the objective lenses (it is shaped like a tube; thus, its name). The diaphragm controls the diameter of the cone of light.
18.
What is another name for the bright field microscope?
Correct Answer
B. Compound microscope
Explanation
A simple microscope has only one lens. A compound microscope utilizes two lenses: an ocular lens and an objective lens. The compound microscope is also referred to as a "light microscope" or "bright field microscope". A phase contrast microscope is useful for examining living cells, because the specimen does not need to be stained. A dissection microscope uses low power magnification. Things appear three dimensional with a dissection microscope. A transmission electron microscope does not use light, but rather a beam of electrons.
19.
Which microscope would be particularly useful for looking at living cells?
Correct Answer
C. pHase contrast microscope
Explanation
A simple microscope has only one lens. A compound microscope utilizes two lenses: an ocular lens and an objective lens. The compound microscope is also referred to as a "light microscope" or "bright field microscope". A phase contrast microscope is useful for examining living cells, because the specimen does not need to be stained. A dissection microscope uses low power magnification. Things appear three dimensional with a dissection microscope. A transmission electron microscope does not use light, but rather a beam of electrons.
20.
Which type of microscope has only one lens?
Correct Answer
A. Simple microscope
Explanation
A simple microscope has only one lens. A compound microscope utilizes two lenses: an ocular lens and an objective lens. The compound microscope is also referred to as a "light microscope" or "bright field microscope". A phase contrast microscope is useful for examining living cells, because the specimen does not need to be stained. A dissection microscope uses low power magnification. Things appear three dimensional with a dissection microscope. A transmission electron microscope does not use light, but rather a beam of electrons.
21.
Which of the following stain blue with H&E stain?
Correct Answer
C. Nucleus
Explanation
"H&E" stands for hematoxylin and eosin. Hematoxylin can be thought of as a basic dye. It binds to acidic structures, staining them blue to purple. For example, it will bind and stain nucleic acids. Therefore, the nucleus stains blue.
22.
What are Sudan stains used primarily for?
Correct Answer
B. Fat
Explanation
Sudan stains are used for staining of lipids and phospholipids. Examples of such stains are sudan black and sudan IV. Oil red O is another stain used for demonstrating fat.
23.
What is Mucicarmine stain used primarily for?
Correct Answer
E. Epithelial mucin
Explanation
Mucicarmine is used to see epithelial mucin. It stains it a deep red.
24.
What is Wright's stain used primarily for?
Correct Answer
A. Blood
Explanation
Wright's stain is used for blood smears and bone marrow. Red blood cells will stain pink.
25.
What color do elastic fibers stain with Verhoeff Elastic stain?
Correct Answer
D. Blue/black
Explanation
Verhoeff Elastic stain stains elastic fibers blue/black. Collagen stains pink/red.
26.
During the preparation of a routine H&E slide, what step occurs after the tissue is preserved?
Correct Answer
E. Dehydration
Explanation
After the tissue is preserved, the next step in the preparation of a routine H&E slide is dehydration. Dehydration involves removing water from the tissue by gradually increasing the concentration of alcohol. This step is necessary to prepare the tissue for embedding in paraffin, which is the subsequent step in the process. Dehydration helps to remove water from the tissue, making it easier to impregnate the tissue with paraffin wax.
27.
During the preparation of a routine H&E slide, how is the tissue preserved?
Correct Answer
A. Fixation
Explanation
During the preparation of a routine H&E slide, the tissue is preserved through fixation. Fixation involves treating the tissue with a chemical solution, such as formalin, to prevent decay and preserve the cellular structure. This process helps to maintain the integrity of the tissue and prevent any changes or degradation that may occur during subsequent steps of the slide preparation. Fixation is an essential step in histology as it allows for accurate examination and analysis of the tissue under a microscope.
28.
During the preparation of a routine H&E slide, what allows the tissue to be visualized?
Correct Answer
C. Staining
Explanation
There are several steps in the preparation of a slide for microscopic examination. First, the tissue is "fixed" with formalin. This preserves it. After the tissue is fixed, it is dehydrated (dehydration will occur twice during slide preparation). The tissue sample is then embedded in paraffin. This forms a solid block, so that it can be placed in a microtome. It is then thinly sliced using a microtome. A thin slice of the specimen is then placed on a slide. The paraffin is removed by dissolving it with a chemical. The tissue is then rehydrated. In order to view the tissue, it is stained. After it is stained, it is again dehydrated. A coverslip is the mounted on top of it. The specimen is then ready for viewing.
29.
Which of the following would be best suited to visualize reticular fibers?
Correct Answer
D. Silver impregnation
Explanation
Silver impregnation is the best suited method to visualize reticular fibers. Reticular fibers are delicate and fine fibers that form a network within connective tissues. They are difficult to visualize using routine staining methods. Silver impregnation techniques involve the deposition of silver salts onto the reticular fibers, making them visible under a microscope. This staining method specifically targets and highlights reticular fibers, allowing for their clear visualization and study.
30.
Which of the following would be best suited to visualize lipid?
Correct Answer
C. Sudan stain
Explanation
Sudan stain would be best suited to visualize lipids because it is a specific stain that binds to lipids, making them visible under a microscope. This stain is commonly used in histology to identify and study lipid-rich tissues or structures, such as adipose tissue. It allows for the visualization and differentiation of lipids based on their color or intensity of staining, providing valuable information about their distribution and composition.
31.
During the preparation of a routine H&E slide, what allows the tissue to hold its form?
Correct Answer
B. Embedding in paraffin
Explanation
Embedding in paraffin allows the tissue to hold its form during the preparation of a routine H&E slide. Paraffin embedding involves immersing the tissue in liquid paraffin, which then solidifies, providing support and preserving the tissue structure. This process helps to maintain the integrity of the tissue and allows for thin and consistent slicing of the sample for microscopic examination. Fixation, staining, and dehydration are important steps in the slide preparation process, but they do not directly contribute to the tissue's ability to hold its form.
32.
During the preparation of a routine H&E slide, what step occurs after the tissue is embedded in paraffin?
Correct Answer
D. Slicing
Explanation
After the tissue is embedded in paraffin, the next step in the preparation of a routine H&E slide is slicing. Slicing refers to the process of cutting thin sections of the tissue using a microtome. These thin sections are then placed on glass slides for further processing and staining.
33.
During the preparation of a routine H&E slide, what step occurs after the tissue is stained?
Correct Answer
E. Dehydration
Explanation
There are several steps in the preparation of a slide for microscopic examination. First, the tissue is "fixed" with formalin. This preserves it. After the tissue is fixed, it is dehydrated (dehydration will occur twice during slide preparation). The tissue sample is then embedded in paraffin. This forms a solid block, so that it can be placed in a microtome. It is then thinly sliced using a microtome. A thin slice of the specimen is then placed on a slide. The paraffin is removed by dissolving it with a chemical. The tissue is then rehydrated. In order to view the tissue, it is stained. After it is stained, it is again dehydrated. A coverslip is then mounted on top of it. The specimen is then ready for viewing.
34.
Which of the following is an acid dye?
Correct Answer
D. Eosin
Explanation
Eosin is an acid dye. It will bind to and stain basic structures (or negatively charged structures), such as cationic amino groups on proteins. It stains them pink. Cytoplasm, muscle, connective tissue, and decalcified bone matrix all stain pink with eosin. "H&E" stand for hematoxylin and eosin. Hematoxylin and eosin stain is used for routine tissue preparation frequently.
Azure II, Toluidine blue, Methylene blue, Thionine are all basic dyes.
35.
Which of the following would be best suited for peripheral blood smears?
Correct Answer
A. Wright's stain
Explanation
Wright's stain would be best suited for peripheral blood smears because it is a combination of eosin and methylene blue dyes that allows for differentiation of different types of blood cells. It stains the cytoplasm of red blood cells pink, the nuclei of white blood cells purple, and the granules in certain white blood cells blue or purple. This staining pattern allows for easy identification and differentiation of different blood cell types under a microscope.
36.
Which of the following stains is used for routine histological examination?
Correct Answer
B. Hematoxylin and eosin stain
Explanation
A peripheral blood smear would be best visualized with Wright's stain. Hematoxylin and eosin stain is the most commonly used tissue stain for routine histological examination. Lipids are best displayed with a sudan stain. Silver impregnation, such as with a reticular stain, can be used to visualize reticular fibers. Collagen fibers can be differentiated from other fibers by staining with Masson's trichrome stain.
37.
What is Prussian blue stain used for?
Correct Answer
E. Iron
Explanation
Prussian blue stain is used to stain iron.
38.
What color does hematoxylin stain structures?
Correct Answer
B. Blue
Explanation
"H & E" stands for hematoxylin and eosin. Hematoxylin can be thought of as a basic dye. It binds to acidic structures, staining them blue to purple. For example, it will bind and stains nucleic acids. Therefore, the nucleus stains blue.
39.
Which of the following would be best suited to differentiate collagen fibers from other fibers ?
Correct Answer
E. Masson's trichrome stain
Explanation
A peripheral blood smear would be best visualized with Wright's stain. Hematoxylin and eosin stain is the most commonly used tissue stain for routine histological examination. Lipids are best displayed with a sudan stain. Silver impregnation, such as with a reticular stain, can be used to visualize reticular fibers. Collagen fibers can be differentiated from other fibers by staining with Masson's trichrome stain.
40.
Which of the following is NOT a membranous organelle?
Correct Answer
D. Ribosomes
Explanation
Lysosomes, peroxisomes, mitochondria, and endoplasmic reticulum are all membranous organelles.
41.
Which organelle contains digestive enzymes?
Correct Answer
C. Lysosome
Explanation
Lysosomes are membrane bound sacs of digestive enzymes. They are nicknamed "suicide sacs".
42.
Which organelle is considered the powerhouse of the cell?
Correct Answer
E. Mitochondria
Explanation
The mitochondria is the site of ATP production for the cell. ATP is the energy currency of the cell. Mitochondria are nicknamed, the "powerhouse".
43.
What structure is at the base of cilia?
Correct Answer
D. Centrioles
Explanation
Centrioles are composed of microtubules. Centrioles are at the base of cilia and flagella. They are also involved with cell division.
44.
Which of the following is NOT considered an inclusion?
Correct Answer
E. Mitochondria
Explanation
Mitochondria is an organelle. Pigment, glycogen, lipid, and secretory granules are all considered inclusions. Organelles perform an activity; they have a job; they require energy. Inclusions are for storage. Inclusions do not perform an energy requiring job.
45.
Collectively, what is the cytoplasm and nucleus called?
Correct Answer
C. Protoplasm
Explanation
The cell boundary is defined by the cell membrane or plasmalemma. Glycocalyx is a polysacchride that can be found on the outer surface of the cell membranes. Collectively, the cytoplasm and nucleus form the protoplasm of a cell. The folds on the inner mitochodrial membrane are called cristae. The cytoplasm that surrounds organelles is the cytoplasmic ground substance or cytosol.
46.
How thick is the plasma membrane?
Correct Answer
B. 8-10 nanometers
Explanation
The plasma membrane is 8-10 nanometers thick.
47.
What is the term for the general process that cells us to expunge material from the cell?
Correct Answer
B. Exocytosis
Explanation
Endotyosis is the term for the process cells use to have substances enter them. Exoctyosis is the term for the process cells use to remove substances. Phagocytosis is "cell eating" and is used for the transport of particulate matter. Pinocytosis is "cell drinking". Active transport is a method which requires energy.
48.
Which organelle sorts and packages proteins within a cell?
Correct Answer
D. Golgi apparatus
Explanation
The Golgi apparatus are stacks of membranous sacs. Proteins are sorted and packaged in the Golgi apparatus.
49.
Which of the following is NOT a membranous organelle?
Correct Answer
B. Filaments
Explanation
Lysosomes, peroxisomes, mitochondria, and endoplasmic reticulum are all membranous organelles.
50.
What are the folds on the inner mitochodrial membrane called?
Correct Answer
D. Cristae
Explanation
The cell boundary is defined by the cell membrane or plasmalemma. Glycocalyx is a polysacchride that can be found on the outer surface of the cell membranes. Collectively, the cytoplasm and nucleus form the protoplasm of a cell. The folds on the inner mitochodrial membrane are called cristae. The cytoplasm that surrounds organelles is the cytoplasmic ground substance or cytosol.