Micro Practice Practical For Perryman Lab
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- 1.
Many Bacteria and Fungi Produce what that they release into their enviroment?
Explanation
Page 161
These Exoenzymes act specifically on particular substrates, degrading macromolecules, such as polysaccharides, protein, DNA, and lipids.Rate this question:
- 2.
What enzyme hydrolyzes the substrate starch?
- A.
Amylase
- B.
DNase
- C.
Collagenase
- D.
Caseinase
- E.
Lipase
Correct Answer
A. AmylaseExplanation
Page 161Rate this question:
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- 3.
What enzyme hydrolyzes the substrate gelatin?
- A.
Amylase
- B.
DNase
- C.
Collagenase
- D.
Caseinase
- E.
Lipase
Correct Answer
C. CollagenaseExplanation
Page 161Rate this question:
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- 4.
What enzyme hydrolyzes the casein protein?
- A.
Amylase
- B.
DNase
- C.
Collagenase
- D.
Caseinase
- E.
Lipase
Correct Answer
D. CaseinaseExplanation
Page 161Rate this question:
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- 5.
What enzyme hydrolyzes the breakdown of tributyrin?
- A.
Amylase
- B.
DNase
- C.
Collagenase
- D.
Caseinase
- E.
Lipase
Correct Answer
E. LipaseExplanation
Page 161Rate this question:
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- 6.
Startch Hydrolysis can be tested with
- A.
The IMViC series.
- B.
A starch agar plate.
- C.
A DNA agar plate.
- D.
A Gram Stain.
- E.
A tube of gelatin agar, and Gram's iodine.
Correct Answer
B. A starch agar plate.Explanation
Page 162Rate this question:
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- 7.
Gram's Iodine is added to media after incubation to determine the presence of DNase.
- A.
True
- B.
False
Correct Answer
B. FalseExplanation
Page 162
Gram's Iodine is added to media after incubation to determine the presence of Amylace.Rate this question:
-
- 8.
DNA Hydrolysis can be tested with
- A.
The IMViC series.
- B.
A starch agar plate.
- C.
A DNA agar plate.
- D.
A Gram Stain.
- E.
A tube of gelatin agar, and Gram's iodine.
Correct Answer
C. A DNA agar plate.Explanation
Page 163Rate this question:
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- 9.
After adding Gram's Iodine starch agar turns purple unless there is a presence of this enzyme
- A.
Amylase
- B.
DNase
- C.
Collagenase
- D.
Caseinase
- E.
Lipase
Correct Answer
A. AmylaseExplanation
Page 163Rate this question:
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- 10.
After innoculating an organism in Starch agar and adding Gram's Iodine, if the organism has a presence of amylase the starch agar will turn what color?
- A.
Purple
- B.
Red
- C.
Yellow
- D.
Metallic Green
- E.
None of the others
Correct Answer
E. None of the othersExplanation
Page 163
If amylase is present than the starch will be broken down leaving the agar colorless.Rate this question:
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- 11.
If you are innoculating as organism that has a presence of DNase in DNA agar, the agar will turn what color?
- A.
Off white
- B.
Pale Green
- C.
Methyl Green
- D.
Metallic Green
- E.
Clear
Correct Answer
E. ClearExplanation
Page 164
If DNase is present than the DNA will be broken down leaving the agar colorless.Rate this question:
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- 12.
After Innoculating in DNA agar, if the agar is methly green around the organism, the results are?
- A.
Positive
- B.
Negative
- C.
Contaminated
- D.
False Positive
Correct Answer
B. NegativeExplanation
Page 164
If DNase is present than the DNA will be broken down leaving the agar colorless.Rate this question:
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- 13.
After Innoculating in Starch agar, and adding Gram's Iodine, if the agar is clear around the organism, the results are?
- A.
Positive
- B.
Negative
- C.
Contaminated
- D.
False Positive
Correct Answer
A. PositiveExplanation
Page 162
If Amylase is present than the Starch will be broken down leaving the agar colorless.Rate this question:
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- 14.
Starch agars is a Selective Media.
- A.
True
- B.
False
Correct Answer
B. FalseExplanation
Page 162
Starch agar detects the presence of Amylace but any organism will be able to grow on Starch agar, meaning that it is NOT Selective.Rate this question:
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- 15.
In Gelatin Hydrolosis if the organism and media become liquid this means that the organism is positive for Collagenase.
- A.
True
- B.
False
Correct Answer
A. TrueExplanation
Page. 164-165Rate this question:
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- 16.
After Innoculation, Serratia marcescens...
- A.
Has a red pigment.
- B.
Has a metallic green sheen color.
- C.
Resembles a black caterpillar.
- D.
Must have phenol red dye added to it to see it.
Correct Answer
A. Has a red pigment.Explanation
Page. 164
fig. 19.3Rate this question:
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- 17.
Collagenase can be detected by use of Gelatin agar.
- A.
True
- B.
False
Correct Answer
A. TrueExplanation
Page 164Rate this question:
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- 18.
Can any organism grow in differential media?
- A.
Yes
- B.
No
Correct Answer
A. YesExplanation
Differential media will allow all organisms to grow.Rate this question:
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- 19.
PEA is selective for what Gram___________ Organisms
- A.
Positive
- B.
Negative
Correct Answer
A. PositiveExplanation
p. 172Rate this question:
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- 20.
EMB is selective for what Gram___________ Organisms
- A.
Positive
- B.
Negative
Correct Answer
B. NegativeExplanation
p. 172Rate this question:
-
- 21.
PEA containes
- A.
Phenylethyl alcohol
- B.
Phenol red
- C.
Potassium eosin acids
- D.
Eosin and methylene blue
Correct Answer
A. pHenylethyl alcoholExplanation
p. 172Rate this question:
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- 22.
EMB containes
- A.
Phenylethyl alcohol
- B.
Phenol red
- C.
Potassium eosin acids
- D.
Eosin and methylene blue
Correct Answer
D. Eosin and methylene blueExplanation
p. 172Rate this question:
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- 23.
Which media is not only selective but differental as well?
- A.
PEA
- B.
EMB
- C.
PIE
- D.
ERA
Correct Answer
B. EMBExplanation
EMB has lactose which detects lactose fermentation in EMB media.Rate this question:
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- 24.
If an organism has growth on a PEA plate it is
- A.
Gram Positive
- B.
Gram Negative
- C.
Staphylococcus
- D.
Streptococcus
Correct Answer
A. Gram PositiveExplanation
p 174Rate this question:
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- 25.
If an organism has growth on a EMB plate it is
- A.
Gram Positive
- B.
Gram Negative
- C.
Staphylococcus
- D.
Streptococcus
Correct Answer
B. Gram NegativeExplanation
p 174Rate this question:
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- 26.
Media: EMB Plate Results: Organism has no growth. Which of the following can you interpreate from the results about the organism?
- A.
The organism is Gram Negative
- B.
The organism is Gram Positive
- C.
The organism is Gram Negative and Ferments Lactose
- D.
The organism is E.Coli
- E.
The Organism is Gram Positive and Ferments Lactose
- F.
None of the others
Correct Answer
B. The organism is Gram PositiveExplanation
P 174Rate this question:
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- 27.
Media: PEA Plate Results: Organism has no growth. Which of the following can you interpreate from the results about the organism?
- A.
The organism is Gram Negative
- B.
The organism is Gram Positive
- C.
The organism is Gram Negative and Ferments Lactose
- D.
The organism is E.Coli
- E.
The Organism is Gram Positive and Ferments Lactose
- F.
None of the others
Correct Answer
A. The organism is Gram NegativeExplanation
P 174Rate this question:
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- 28.
Media: PEA Plate Results: Organism has growth. Which of the following can you interpreate from the results about the organism?
- A.
The organism is Gram Negative
- B.
The organism is Gram Positive
- C.
The organism is Gram Negative and Ferments Lactose
- D.
The organism is E.Coli
- E.
The Organism is Gram Positive and Ferments Lactose
- F.
None of the others
Correct Answer
B. The organism is Gram PositiveExplanation
P 174Rate this question:
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- 29.
Media: EMB Plate Results: Organism has growth. Which of the following can you interpreate from the results about the organism?
- A.
The organism is Gram Negative
- B.
The organism is Gram Positive
- C.
The organism is Gram Negative and Ferments Lactose
- D.
The organism is E.Coli
- E.
The Organism is Gram Positive and Ferments Lactose
- F.
None of the others
Correct Answer
A. The organism is Gram NegativeExplanation
P 174Rate this question:
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- 30.
Media: EMB Plate Results: Organism has Metalic Green Sheen. Which of the following can you interpreate from the results about the organism?
- A.
The organism is Gram Negative
- B.
The organism is Gram Positive
- C.
The organism is Gram Negative and Ferments Lactose
- D.
The organism is E.Coli
- E.
The Organism is Gram Positive and Ferments Lactose
- F.
None of the others
Correct Answer
D. The organism is E.ColiExplanation
P 174Rate this question:
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- 31.
Media: EMB Plate Results: Organism has growth with dark circles. Which of the following can you interpreate from the results about the organism?
- A.
The organism is Gram Negative
- B.
The organism is Gram Positive
- C.
The organism is Gram Negative and Ferments Lactose
- D.
The organism is E.Coli
- E.
The Organism is Gram Positive and Ferments Lactose
- F.
None of the others
Correct Answer
C. The organism is Gram Negative and Ferments LactoseExplanation
P 174Rate this question:
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- 32.
Media: MSA Plate Results: No Growth Which of the following can you interpreate from the results about the organism?
- A.
Staphylococcus
- B.
Streptococcus
- C.
Staphylococcus Ferments Mannitol
- D.
Streptococcus Ferments Mannitol
- E.
None of the others
Correct Answer
E. None of the othersExplanation
P 180
No Growth means the Organism is NOT StaphylococcusRate this question:
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- 33.
Media: MSA Plate Results: Growth Which of the following can you interpreate from the results about the organism?
- A.
Staphylococcus
- B.
Streptococcus
- C.
Staphylococcus Ferments Mannitol
- D.
Streptococcus Ferments Mannitol
- E.
None of the others
Correct Answer
A. StapHylococcusExplanation
P 180Rate this question:
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- 34.
Media: MSA Plate Results: Growth with Yellowing of the Medium Which of the following can you interpreate from the results about the organism?
- A.
Staphylococcus
- B.
Streptococcus
- C.
Staphylococcus Ferments Mannitol
- D.
Streptococcus Ferments Mannitol
- E.
None of the others
Correct Answer
C. StapHylococcus Ferments MannitolExplanation
P 180Rate this question:
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- 35.
MSA is
- A.
Selective Media
- B.
Differential Media
- C.
Selective and Differential Media
- D.
None of the others
Correct Answer
C. Selective and Differential MediaExplanation
Selective and differential media (SD media) is a type of culture medium that is designed to selectively promote the growth of certain microorganisms while inhibiting the growth of others. It contains specific nutrients or chemicals that favor the growth of particular organisms, allowing them to grow while suppressing the growth of other organisms. Additionally, SD media also contains indicators that can differentiate between different types of microorganisms based on their metabolic activities or biochemical characteristics. Therefore, MSA (Mannitol Salt Agar) can be classified as both selective and differential media as it selects for the growth of salt-tolerant bacteria and differentiates between bacteria based on their ability to ferment mannitol.Rate this question:
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- 36.
MSA stands for
- A.
Mannitol Salt Algar
- B.
Media Sulfur Acid
- C.
My Sweet Alex
- D.
MultiSellular Actins
Correct Answer
A. Mannitol Salt Algar -
- 37.
MSA identifies mmembers of
- A.
Streptococcus
- B.
Staphylococcus
- C.
Bacillus
- D.
Gram Positive
Correct Answer
B. StapHylococcusExplanation
MSA (Mannitol Salt Agar) is a selective and differential medium used to identify and isolate Staphylococcus species. It contains high salt concentration, which inhibits the growth of most bacteria except for Staphylococcus, which can tolerate high salt levels. The medium also contains mannitol sugar, which is fermented by certain Staphylococcus species, causing a change in the pH of the agar and resulting in a yellow color change. Therefore, the presence of yellow colonies on MSA indicates the presence of Staphylococcus.Rate this question:
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- 38.
What does Coagulase do?
- A.
Breaks down Enzyme Coage
- B.
Clots Plasma
- C.
Detects Gram Positive Organisms
- D.
Detects Gram Negative Organisms
Correct Answer
B. Clots PlasmaExplanation
Coagulase is an enzyme produced by certain bacteria that promotes blood clotting. When present, coagulase causes the conversion of fibrinogen to fibrin, resulting in the formation of a clot. This ability is important for the bacteria's survival as it helps them to evade the host immune response by forming a protective barrier around themselves. Therefore, the correct answer is "Clots Plasma".Rate this question:
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- 39.
Staphylococcus aureus is the only Staph that has
- A.
Teticohalogen
- B.
Coagulase
- C.
Leukocidin
- D.
Staphylokinase
Correct Answer
B. CoagulaseExplanation
Coagulase is an enzyme produced by Staphylococcus aureus that is responsible for the formation of fibrin clots. This ability is unique to Staphylococcus aureus and is not found in other staphylococcal species. Coagulase plays a crucial role in the pathogenesis of Staphylococcus aureus infections by helping the bacteria evade the host immune response and form protective barriers.Rate this question:
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- 40.
How Does MSA Work? What makes it Selective?
- A.
7.5% NaCl
- B.
Ph balance of 6.8
- C.
PH indicator Phenol Red
- D.
Mannitol
Correct Answer
A. 7.5% NaClExplanation
The correct answer is 7.5% NaCl. This solution is selective because it contains a high concentration of salt (NaCl). This high salt concentration creates a hypertonic environment, which inhibits the growth of most bacteria. Only halophilic (salt-loving) bacteria are able to survive and grow in this selective medium.Rate this question:
-
- 41.
How Does MSA Work? What makes it Differential?
- A.
7.5% NaCl
- B.
Ph balance of 6.8
- C.
PH indicator Phenol Red
- D.
Mannitol
Correct Answer
D. MannitolExplanation
Mannitol is the component that makes MSA (Mannitol Salt Agar) differential. MSA is a selective and differential medium used for the isolation and differentiation of Staphylococcus aureus from other staphylococci. The high concentration of salt (7.5% NaCl) in MSA inhibits the growth of most bacteria except for staphylococci. Staphylococcus aureus, which can ferment mannitol, produces acid that lowers the pH of the medium, causing a color change in the pH indicator phenol red from red to yellow. Other staphylococci that cannot ferment mannitol do not produce acid and the medium remains red. Therefore, the ability to ferment mannitol differentiates S. aureus from other staphylococci.Rate this question:
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- 42.
MSA has a pH indicator, if the agar turns yellow, the results should be interpreted as
- A.
Positive for Mannitol
- B.
Negative for Mannitol
- C.
Positive for NaCl
- D.
Negative for NaCl
Correct Answer
A. Positive for MannitolExplanation
If the MSA agar turns yellow, it indicates a low pH level. This change in color suggests that the bacteria present on the agar have fermented the mannitol sugar, producing acid as a byproduct. Therefore, the result is interpreted as positive for mannitol fermentation.Rate this question:
-
- 43.
What enzyme degrades hydrogen peroxide to water and oxygen gas?
Correct Answer
Catalase
catalase
catlase
Catlase
Catalace
CatalaceExplanation
Catalase is the correct answer for this question. Catalase is an enzyme that is found in cells and helps to break down hydrogen peroxide into water and oxygen gas. It plays a crucial role in protecting cells from the harmful effects of hydrogen peroxide, which is a byproduct of various metabolic processes. By catalyzing the decomposition of hydrogen peroxide, catalase ensures that it is converted into harmless substances, preventing any damage to the cell.Rate this question:
- 44.
Catalase Test causes what reaction in Staphs?
- A.
Bubbling
- B.
Smoking
- C.
Stringing
- D.
Color Change
- E.
None of the others
Correct Answer
A. BubblingExplanation
The catalase test causes bubbling in Staphs. This reaction occurs because catalase is an enzyme that breaks down hydrogen peroxide into water and oxygen. When hydrogen peroxide is added to a sample containing Staphylococci, the catalase enzyme present in the bacteria catalyzes the breakdown of hydrogen peroxide, resulting in the release of oxygen gas. This release of oxygen gas causes bubbling to occur, indicating a positive catalase test.Rate this question:
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- 45.
Catalase Test causes what reaction in Streps?
- A.
Bubbling
- B.
Smoking
- C.
Stringing
- D.
Color Change
- E.
None of the others
Correct Answer
E. None of the othersExplanation
Catalase has No reaction for StrepsRate this question:
-
- 46.
Catalase is a fast test to determine whether an organism is a Staph or Strep
- A.
True
- B.
False
Correct Answer
A. TrueExplanation
Catalase is indeed a fast test that can be used to differentiate between Staphylococcus and Streptococcus organisms. This test involves adding hydrogen peroxide to a bacterial culture, and if the bacteria produce the enzyme catalase, they will break down the hydrogen peroxide into water and oxygen, resulting in the formation of bubbles. Staphylococcus species produce catalase, while Streptococcus species do not. Therefore, the statement that catalase is a fast test to determine whether an organism is a Staph or Strep is true.Rate this question:
-
- 47.
Staphlycoccus are Gram Positive Cocci
- A.
True
- B.
False
Correct Answer
B. FalseExplanation
p 189Rate this question:
-
- 48.
Streptococcus are Gram Positive Cocci
- A.
True
- B.
False
Correct Answer
A. TrueExplanation
p 189Rate this question:
-
- 49.
Which is Halophilic
- A.
Streptococcus
- B.
Staphylococcus
Correct Answer
B. StapHylococcusExplanation
Staphylococcus is halophilic because it is able to tolerate high salt concentrations. This bacterium can survive and thrive in environments with high salt content, such as salty foods or the human skin. Streptococcus, on the other hand, is not halophilic and does not have the ability to tolerate high salt concentrations.Rate this question:
-
- 50.
Hemolysis is
- A.
Lysis of red blood cells.
- B.
Lysis of whit blood cells.
- C.
Lysis of muscle cells.
- D.
Lysis of delta cells.
Correct Answer
A. Lysis of red blood cells.Explanation
Hemolysis refers to the breakdown or destruction of red blood cells. This process can occur due to various factors such as infections, toxins, or certain medical conditions. When red blood cells undergo hemolysis, their membranes rupture, leading to the release of their contents into the surrounding fluid. This can result in the release of hemoglobin, which gives the characteristic red color to blood, and other cellular components. Hemolysis can have detrimental effects on the body, including anemia and the release of substances that can cause inflammation and damage to tissues.Rate this question:
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Dec 14, 2023Quiz Edited by
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Nov 09, 2011Quiz Created by
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