Microbiology Chapter 2 Quiz Questions

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Quizzes Created: 12 | Total Attempts: 7,232

Questions: 62 | Attempts: 950 | Updated: Mar 22, 2023

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Microbiology Chapter 2

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Mar 22, 2023

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May 23, 2012

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Microbiology Chapter 2 Quiz Questions

The __________ is the point at which a lens focuses parallel beams of light.

The __________ is the distance between the center of a lens and the point at which it focuses parallel beams of light.

Light rays are refracted (bent) when they cross the interface between materials with different refractive indices.

Which of these microscopes can be used to create high-resolution three-dimensional images of cells?

Confocal microscopes exhibit improved contrast and resolution by

A 30 objective and a 20 ocular produce a total magnification of

A 45 objective and a 10 ocular produce a total magnification of

A microscope that exposes specimens to ultraviolet, violet, or blue light and forms an image with the light emitted at a different wavelength is called a __________ microscope.

Immersion oil can be used to increase the resolution achieved with some microscope lenses because it increases the __________ between the specimen and the objective lens.

A substage condenser is used to focus light onto the specimen, which increases the resolution of a light microscope.

The __________ is the distance between the specimen and the objective lens when the specimen is in focus.

The useful magnification of a light microscope is limited by the ___________ of the light source being utilized.

The special dyes used in fluorescence microscopy that absorb light at one wavelength and emit light at a different wavelength are called __________.

In order to view a specimen with a total magnification of 400, a __________ objective must be used if the ocular is 10.

Confocal microscopes, in combination with specialized computer software, can be used to create three-dimensional images of cell structures.

A light microscope with an objective lens numerical aperture of 0.65 is capable of allowing two objects 400 nm apart to be distinguished when using light with a wavelength of 420 nm.

Resolution decreases when the wavelength of the illuminating light decreases.

Immersion oil is used to prevent a specimen from drying out.

It is possible to build a light microscope capable of 10,000 magnification, but the image would not be sharp because resolution is independent of magnification.

Immersion oil increases the amount of light passing through a specimen and entering the objective lens.

If the objective lenses of a microscope can be changed without losing focus on the specimen, they are said to be

An instrument that magnifies slight differences in the refractive index of cell structures is called a (n) __________ microscope.

The instrument that produces a bright image of the specimen against a dark background is called a (n) __________ microscope.

As the magnification of a series of objective lenses increases, the working distance

Prior to staining, smears of microorganisms are heat-fixed in order to

Acid-fast organisms such as Mycobacterium tuberculosis contain __________ constructed from mycolic acids in their cell walls.

In the Gram-staining procedure, the primary stain is

In the Gram-staining procedure, the decolorizer is

In the Gram-staining procedure, the counterstain is

In the Gram-staining procedure, the mordant is

After the primary stain has been added but before the decolorizer has been used, gram positive organisms are stained __________ and gram-negative organisms are stained __________.

After the decolorizer has been added, gram-positive organisms are stained __________ and gram-negative organisms are stained __________.

After the secondary stain has been added, gram-positive organisms are stained __________ and gram-negative organisms are stained __________.

If the decolorizer is left on too long in the Gram-staining procedure, gram-positive organisms will be stained __________ and gram-negative organisms will be stained __________.

If the decolorizer is not left on long enough in the Gram-staining procedure, gram-positive organisms will be stained __________ and gram-negative organisms will be stained __________.

Which of the following is considered to be a differential staining procedure?

Basic dyes such as methylene blue bind to cellular molecules that are

The Schaeffer-Fulton procedure is used to stain

Gram staining divides bacterial species into roughly two equal groups.

Negative staining facilitates the visualization of bacterial capsules which are intensely stained by the procedure.

Negative staining with India ink can be used to reveal the presence of capsules that surround bacterial cells.

Mordants increase the binding between a stain and specimen.

In order to stain flagella so that they may be readily observed by light microscopy, it is usually necessary to increase their thickness.

The procedure in which a single stain is used to visualize microorganisms is called __________ staining.

__________ is the process by which internal and external structures of cells and organisms are preserved and maintained in position.

Thin films of bacteria that have been air-dried onto a glass microscope slide are called __________.

A procedure that divides organisms into two or more groups depending on their individual reactions to the same staining procedure is referred to as __________ staining.

The Gram-staining procedure is an example of:

The Gram-staining procedure is widely used because it allows rapid identification of a microorganism with little additional testing.

Regions of a specimen with higher electron density scatter ___________ electrons and, therefore, appear __________ in the image projected onto the screen of a transmission electron microscope.

After the primary stain has been added but before the decolorizer has been used, gram positive organisms are stained and gram-negative organisms are stained .

After the decolorizer has been added, gram-positive organisms are stained and gram-negative organisms are stained .

After the secondary stain has been added, gram-positive organisms are stained and gram-negative organisms are stained .

If the decolorizer is left on too long in the Gram-staining procedure, gram-positive organisms will be stained and gram-negative organisms will be stained .

If the decolorizer is not left on long enough in the Gram-staining procedure, gram-positive organisms will be stained and gram-negative organisms will be stained .

Regions of a specimen with higher electron density scatter _ electrons and, therefore, appear in the image projected onto the screen of a transmission electron microscope.