Microbiology MCQ Trivia Questions!

Turns yellow = positive, remains green = negative.

It is a green coloured solid enriched media in a test tube, used for TBC.

for answers b) c) and d), Hanja medium + MIU is used.

Is used as part of MIU complex test tube test.

VL-broth is used for anaerobes. Sabouraud agar is used for fungi. Slanetz-Bartley is used for enterococci. Selenite broth is an example of a Selectively Multiplying liquid media used to culture Salmonella.

Iit is a direct method. Positive result means presence of the microbe in the patient‘s body.

It is used for pathogenous Neisseriae and Hemophili (that do not grow even on blood agar)

Formation of H2S causes bottom part of medium to turn black.

Example of use: we can read HIT results for tick-born encephalitis.

MIC > breakpoint

= strain is resistant

Turns yellow = positive, remains green = negative.

In the first phase of PCR reaction, the main objective is to find isolated DNA. This implies that the DNA needs to be separated and purified from other cellular components before proceeding with the PCR reaction. The isolation of DNA is a crucial step to ensure accurate and reliable results in PCR.

The catalase test is used for the diagnostics of both Gram-positive cocci and Gram-positive rods. It involves the use of hydrogen peroxide to determine the presence of the enzyme catalase, which breaks down hydrogen peroxide into water and oxygen. Since all the given answers correctly describe the purpose and use of the catalase test, the answer "all answers are correct" is appropriate.

The E-test is quantitative

Only some G- bacteria can grow on it selectively.

ASO examination is indicated in rheumatoid fever. Rheumatoid fever is a condition that can occur after a streptococcal infection, and it is characterized by inflammation in the joints, heart, and other organs. ASO (Anti-Streptolysin O) is a test that measures the level of antibodies in the blood against the streptococcal bacteria. An elevated level of ASO antibodies indicates a recent streptococcal infection, which can be a potential cause of rheumatoid fever. Therefore, performing an ASO examination is recommended in cases of suspected rheumatoid fever to confirm the presence of a streptococcal infection.

Segmental PCR is not considered a classification of PCR because it is not a commonly recognized or widely used term in the field of PCR. Specific PCR, Multiplex PCR, and Universal PCR are all well-established classifications of PCR techniques. Specific PCR refers to the amplification of a specific target sequence, Multiplex PCR involves the simultaneous amplification of multiple target sequences in a single reaction, and Universal PCR is a technique used to amplify a wide range of target sequences.

The amplification product should be detected by gel electrophoresis or ELISA in third phase.

It is a mathematical binary system used in the plate tests., e.g. ENTEROtest16

MIC value is used in the microdilution test.