1.
NADH, FADH, GTP, ATP are:
Correct Answer
B. High energy intermediates
Explanation
NADH, FADH, GTP, and ATP are considered high energy intermediates. These molecules play crucial roles in cellular energy metabolism and are involved in transferring and storing energy in the form of chemical bonds. NADH and FADH are involved in the electron transport chain, where they donate electrons to generate ATP. GTP is a molecule similar to ATP and is used as an energy source in various cellular processes. Overall, these molecules serve as important intermediates in energy transfer and utilization within cells.
2.
ATP ----> ADP + Pi has dG=?
Correct Answer
B. -30.6 kJ/mol
Explanation
The correct answer is -30.6 kJ/mol. This is because the reaction ATP --> ADP + Pi is an exothermic reaction, meaning it releases energy. The negative value of -30.6 kJ/mol indicates that the reaction releases 30.6 kilojoules of energy per mole of ATP converted to ADP and Pi.
3.
What method of energy (ATP) production is anaerobic?
Correct Answer
fermentation
Explanation
Fermentation is a method of energy (ATP) production that occurs in the absence of oxygen, making it an anaerobic process. During fermentation, glucose is broken down into smaller molecules, such as lactic acid or ethanol, and ATP is produced as a result. This process is commonly observed in microorganisms, such as yeast or bacteria, and also occurs in certain cells of the human body, such as muscle cells, when oxygen supply is limited. Fermentation allows for the production of ATP without the need for oxygen, making it an important energy source in anaerobic conditions.
4.
What method of energy (ATP) production is aerobic?
Correct Answer
respiration
Explanation
Aerobic respiration is the method of energy production that requires oxygen. It occurs in the mitochondria of cells and involves the breakdown of glucose to produce ATP (adenosine triphosphate), the main energy currency of cells. During aerobic respiration, glucose is oxidized in a series of chemical reactions, releasing carbon dioxide and water as byproducts. This process is highly efficient and produces a large amount of ATP compared to other methods of energy production.
5.
How many ATP is required in the preparatory phase of glycolysis to produce 2 GAP molecules?
Correct Answer
2
Explanation
In the preparatory phase of glycolysis, one ATP molecule is used to phosphorylate glucose to produce glucose-6-phosphate. Another ATP molecule is then used to convert glucose-6-phosphate to fructose-6-phosphate. These two reactions are necessary to prepare the glucose molecule for further metabolism. Therefore, a total of 2 ATP molecules are required in the preparatory phase to produce 2 GAP (glyceraldehyde-3-phosphate) molecules.
6.
How many pyrvuvates, NADH, ATP do you get per GAP molecule in the energy production phase of glycolysis? (separate with commas, no spaces)
Correct Answer
1,1,2
Explanation
In the energy production phase of glycolysis, one GAP molecule is converted into one pyruvate molecule, which generates one NADH molecule and two ATP molecules. Therefore, the correct answer is 1,1,2.
7.
What does fermentation produce?
Correct Answer
B. NAD+
Explanation
Fermentation is a metabolic process that occurs in the absence of oxygen. It involves the breakdown of glucose to produce energy in the form of ATP. During fermentation, NADH molecules are oxidized to NAD+, which is necessary for the continuation of the process. Therefore, fermentation produces NAD+ as a result of the oxidation of NADH.
8.
What are the inputs of acetyl coA production
Correct Answer(s)
B. NAD+
C. Pyruvate
D. Coa
Explanation
Acetyl CoA production requires three inputs: NAD+, Pyruvate, and CoA. NAD+ is a coenzyme that functions as an electron carrier in cellular respiration, and it is needed to accept electrons during the conversion of pyruvate to acetyl CoA. Pyruvate is a product of glycolysis, and it is converted to acetyl CoA in the presence of NAD+ and CoA. CoA, or coenzyme A, is a molecule that plays a crucial role in various metabolic reactions, including the conversion of pyruvate to acetyl CoA. Together, these three inputs are necessary for the production of acetyl CoA.
9.
How many NADH does Krebs (one cycle) produce?
Correct Answer(s)
3
Explanation
In one cycle of the Krebs cycle, also known as the citric acid cycle, 3 molecules of NADH are produced. NADH is an important molecule in cellular respiration as it carries high-energy electrons to the electron transport chain, where ATP production occurs. The Krebs cycle is a series of chemical reactions that take place in the mitochondria and is a crucial step in the breakdown of glucose to produce energy in the form of ATP. Therefore, the correct answer is 3.
10.
The other products of Krebs cycle (one cycle) are: 2 CO2, 2 GTP, 2 FADH, CoA
Correct Answer
B. False
Explanation
Only 1 FADH
11.
During the ETC: FADH donates electrons to complex I, NADH donates electrons to complex II
Correct Answer
B. False
Explanation
Other way around
12.
The electrons from complex I and II get shuttled off to ______ next
Correct Answer
B. Ubiquinone
Explanation
The electrons from complex I and II are shuttled off to Ubiquinone next. Ubiquinone, also known as coenzyme Q10, is a mobile electron carrier that plays a crucial role in the electron transport chain. It accepts electrons from complex I and II and transfers them to complex III, facilitating the flow of electrons and the generation of ATP.
13.
Where does the electron transport chain occur in eukaryotes?
Correct Answer
B. Between the mitochondrial membranes (H+ pumped into intermembrane space)
Explanation
The electron transport chain occurs between the mitochondrial membranes in eukaryotes. This is where the process of oxidative phosphorylation takes place, which generates ATP by transferring electrons from NADH and FADH2 to oxygen. As electrons are passed along the chain, protons (H+) are pumped into the intermembrane space, creating a proton gradient that drives ATP synthesis. This location allows for efficient ATP production within the mitochondria, which is the powerhouse of the cell.
14.
Where does the electron transport chain occur in prokaryotes?
Correct Answer
A. The cell membrane
Explanation
In prokaryotes, the electron transport chain occurs in the cell membrane. This is where the proteins and enzymes involved in the electron transport chain are embedded. The electron transport chain is responsible for generating ATP through oxidative phosphorylation, and it occurs in the inner mitochondrial membrane in eukaryotes. However, prokaryotes lack mitochondria, so their electron transport chain takes place in their cell membrane instead.
15.
What is the enzyme that catalyses ADP + Pi ---> ATP
Correct Answer
ATPase
Explanation
ATPase is the enzyme that catalyzes the reaction of ADP (adenosine diphosphate) and Pi (inorganic phosphate) to form ATP (adenosine triphosphate). This enzyme plays a crucial role in cellular energy metabolism by facilitating the conversion of ADP to ATP, which is the primary energy currency of the cell. ATPase hydrolyzes ATP into ADP and Pi, releasing energy that can be used for various cellular processes. Therefore, ATPase is responsible for the synthesis and breakdown of ATP, regulating the energy balance within the cell.
16.
How many ATP does each NADH produce (ETC)?
Correct Answer
3
Explanation
Each NADH molecule produced during the Electron Transport Chain (ETC) generates 3 molecules of ATP. This is because the NADH donates its electrons to the ETC, which leads to the pumping of protons across the inner mitochondrial membrane. The flow of these protons back through ATP synthase drives the synthesis of ATP. Therefore, each NADH molecule contributes to the production of 3 ATP molecules.
17.
How many ATP does FADH produce (ETC)?
Correct Answer
2
Explanation
FADH produces 2 ATP during the electron transport chain (ETC). FADH is one of the molecules involved in the ETC, specifically in Complex II. It donates its electrons to the electron transport chain, which leads to the pumping of protons across the inner mitochondrial membrane. This proton gradient is then used by ATP synthase to produce ATP. Since FADH donates electrons at a later stage in the ETC compared to NADH, it produces less ATP. NADH, on the other hand, produces 3 ATP molecules during the ETC.
18.
What's the efficiency of aerobic respiration?
Correct Answer
C. 41.5%
Explanation
The efficiency of aerobic respiration is 41.5%. This is because aerobic respiration is a metabolic process that occurs in the presence of oxygen and produces ATP (adenosine triphosphate) molecules, which are the main source of energy for cells. However, not all the energy stored in the glucose molecule is converted into ATP. Some energy is lost as heat during the process, resulting in an efficiency of 41.5%.
19.
What's the total ATP yield for aerobic respiration?
Correct Answer
38
Explanation
The total ATP yield for aerobic respiration is 38. This is because aerobic respiration involves three main steps: glycolysis, the Krebs cycle, and oxidative phosphorylation. In glycolysis, 2 ATP molecules are produced. In the Krebs cycle, 2 ATP molecules are produced directly, and several molecules that can be used to produce more ATP are generated. Finally, in oxidative phosphorylation, the majority of ATP is produced, with a net yield of 34 ATP molecules. Adding up all these ATP molecules gives a total yield of 38 ATP molecules.
20.
What are the phases of cell growth in culture in order?
______ phase, _______ phase, ______ phase, ________phase (separate with commas, no spaces)
Correct Answer
lag,exponential,stationary,death
Explanation
In cell culture, the phases of cell growth occur in a specific order. The first phase is the lag phase, where cells are adjusting to the new environment and preparing for growth. This is followed by the exponential phase, where cells are actively dividing and multiplying at a rapid rate. After reaching a maximum population density, the culture enters the stationary phase, where growth rate slows down and cell division is balanced by cell death. Finally, in the death phase, the number of dying cells exceeds the number of dividing cells, leading to a decline in the overall cell population.
21.
If there are initially 2 cells, the generation time is 1 h/cell, and the culture has been in exponential phase for 12 hours, how many cells are there?
Correct Answer
8192
Explanation
In exponential growth, the number of cells doubles every generation time. Since the generation time is 1 hour per cell, after 12 hours, there would be 2^12 (2 raised to the power of 12) cells. Simplifying this, we get 8192 cells.
22.
As temperature decreases, enzymes function more efficiently
Correct Answer
B. False
Explanation
The statement is false because enzymes function optimally at a specific temperature called the optimum temperature. As the temperature decreases below the optimum temperature, the activity of enzymes decreases, and they become less efficient. Similarly, as the temperature increases above the optimum temperature, enzyme activity also decreases. Therefore, enzymes do not function more efficiently as temperature decreases.
23.
An _______'s ideal environment is where ph<7
Correct Answer
acidophile
Explanation
An acidophile is an organism that thrives in an environment with a pH level below 7. This means that the ideal environment for an acidophile is acidic, as it prefers conditions where the concentration of hydrogen ions (H+) is higher than the concentration of hydroxide ions (OH-). Acidophiles have adapted to survive and function in these acidic conditions, which would be detrimental or even lethal to other organisms that prefer neutral or alkaline environments.
24.
Halophiles need high ______.
Correct Answer
salt
Explanation
Halophiles are organisms that thrive in environments with high salt concentrations. They have adapted to survive and even require high levels of salt to maintain their cellular functions. These organisms have specialized mechanisms to handle the osmotic stress caused by the high salt concentration. The salt provides stability to their cellular structures and helps in maintaining the balance of water and ions within their cells. Therefore, the presence of salt is essential for the growth and survival of halophiles.