Bcid Training - Module 2 Quiz | Attempts: 101

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| By Nicole Siska

Nicole Siska

Community Contributor

Quizzes Created: 4 | Total Attempts: 475

Questions: 13 | Attempts: 101 | Updated: Mar 21, 2023

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Questions and Answers

1.

MALDI can be performed after blood culture without a gram stain result.
- A.
  
  True
- B.
  
  False
Correct Answer
A. True

Explanation
MALDI (Matrix-Assisted Laser Desorption/Ionization) is a technique used for rapid identification of microorganisms in clinical samples. It is commonly used in microbiology laboratories to identify bacteria and fungi directly from positive blood cultures. Gram staining is a method used to classify bacteria into Gram-positive or Gram-negative based on the structure of their cell walls. While it is a useful preliminary test, it is not necessary for performing MALDI. Therefore, MALDI can indeed be performed after blood culture without a gram stain result.

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2.

Traditional blood culture with ID & AST typically takes an average of ____________ hours.
- A.
  
  8-12
- B.
  
  12-24
- C.
  
  24-48
- D.
  
  48-72
- E.
  
  96-120
Correct Answer
D. 48-72

Explanation
Traditional blood culture with ID & AST typically takes an average of 48-72 hours. This is because it takes time for the bacteria or fungi in the blood sample to grow and multiply in the culture medium. After the growth, further tests are conducted to identify the specific microorganism causing the infection and determine its antibiotic susceptibility. This process usually takes a couple of days to complete, hence the 48-72 hour timeframe.

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3.

What technology is used in MALDI-TOF?
- A.
  
  Laser Microdissection
- B.
  
  Reverse Osmosis
- C.
  
  Mass Spectrometry
- D.
  
  Protein Dissolution
- E.
  
  Nuclear magnetic resonance
Correct Answer
C. Mass Spectrometry

Explanation
MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight) is a technology used in Mass Spectrometry. Mass Spectrometry is a technique used to analyze the mass-to-charge ratio of ions in a sample. In MALDI-TOF, a laser is used to ionize and desorb molecules from a sample, which are then accelerated in an electric field and separated based on their mass-to-charge ratio. This technology is commonly used in various fields such as proteomics, drug discovery, and forensic analysis to identify and analyze molecules based on their mass and charge.

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4.

What is detected during the MALDI-TOF process?
- A.
  
  Electrons
- B.
  
  Nucleic acids
- C.
  
  Carbohydrates
- D.
  
  Proteins
Correct Answer
D. Proteins

Explanation
During the MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight) process, proteins are detected. MALDI-TOF is a mass spectrometry technique used to analyze and identify proteins. It involves the ionization of protein samples, followed by their separation based on their mass-to-charge ratio. The resulting mass spectrum provides information about the protein's molecular weight, which can be used to identify the protein and analyze its structure and function. Therefore, proteins are the target analytes detected during the MALDI-TOF process.

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5.

MALDI-TOF results are analyzed by manually reading peaks of the spectrum.
- A.
  
  True
- B.
  
  False
Correct Answer
B. False

Explanation
MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight) results are not manually read by analyzing peaks of the spectrum. Instead, the results are obtained through automated software analysis. This technology uses laser pulses to ionize and desorb molecules from a sample, which are then accelerated and separated based on their mass-to-charge ratio. The resulting spectrum is then processed by specialized software to identify the peaks and determine the molecular composition of the sample. Therefore, the correct answer is False.

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6.

What are the names of the most common MALDI-TOF Systems used in the US for blood culture?
- A.
  
  BioMerieux VITEK MS
- B.
  
  BioMerieux BacT/ALERT® 3D instrument
- C.
  
  Bruker MALDI Biotyper
- D.
  
  Bruker UltrafleXtreme™
- E.
  
  B & c only
- F.
  
  B & d only
- G.
  
  A & c only
- H.
  
  A & d only
Correct Answer
G. A & c only
7.

A sample for MALDI-TOF testing can be performed directly from the blood culture bottle like molecular tests.
- A.
  
  True
- B.
  
  False
Correct Answer
A. True

Explanation
MALDI-TOF testing is a rapid and accurate method used to identify microorganisms in clinical samples. This statement suggests that a sample for MALDI-TOF testing can be taken directly from the blood culture bottle, similar to molecular tests. This is true because MALDI-TOF testing allows for direct identification of microorganisms from the blood culture bottle, without the need for further culturing or isolation steps. This saves time and allows for quicker diagnosis and appropriate treatment of infections.

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8.

What is the average time to result to organism ID using a MALDI-TOF system?
- A.
  
  4-8 hours
- B.
  
  8-12 hours
- C.
  
  12-24 hours
- D.
  
  48-72 hours
- E.
  
  72-96 hours
Correct Answer
C. 12-24 hours

Explanation
The average time to result to organism ID using a MALDI-TOF system is 12-24 hours. This means that on average, it takes between 12 and 24 hours to identify the organism using this system.

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9.

The Bruker MALDI Biotyper is FDA cleared on gram-positive & gram-negative bacterial colonies cultured from human specimens.
- A.
  
  True
- B.
  
  False
Correct Answer
B. False

Explanation
The statement is false because the Bruker MALDI Biotyper is not FDA cleared for gram-positive and gram-negative bacterial colonies cultured from human specimens.

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10.

Lysate preparation or extraction required for some bacteria and yeast is required on the Bruker Biotyper.
- A.
  
  True
- B.
  
  False
Correct Answer
A. True

Explanation
The statement is true because lysate preparation or extraction is indeed required for some bacteria and yeast on the Bruker Biotyper. This process is necessary to obtain the proteins or genetic material from the microorganisms, which can then be analyzed using the Bruker Biotyper system. Lysate preparation involves breaking open the cells to release their contents, allowing for further identification and characterization of the microorganisms.

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11.

For each hour that appropriate targeted antimicrobial treatment is delayed, a sepsis patient’s mortality rate increases by ___%
- A.
  
  4
- B.
  
  8
- C.
  
  12
- D.
  
  24
- E.
  
  48
Correct Answer
B. 8

Explanation
For each hour that appropriate targeted antimicrobial treatment is delayed, a sepsis patient's mortality rate increases by 8%. This means that the longer the delay in administering the treatment, the higher the chances of the patient dying due to sepsis. Prompt and timely administration of antimicrobial treatment is crucial in sepsis cases to effectively combat the infection and prevent further complications.

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12.

Blood Culture ID/AST is commonly performed with MALDI.
- A.
  
  True
- B.
  
  False
Correct Answer
B. False

Explanation
Blood Culture ID/AST is not commonly performed with MALDI. MALDI (Matrix-Assisted Laser Desorption/Ionization) is a technique used in microbiology to identify microorganisms and determine their antibiotic susceptibility. While MALDI is commonly used for microbial identification and antibiotic susceptibility testing, blood culture ID/AST is typically performed using other methods such as automated systems or manual methods like disk diffusion or broth microdilution. Therefore, the statement is false.

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13.

Using MALDI for AST can significantly improve susceptibility TAT compared to traditional methods.
- A.
  
  True
- B.
  
  False
Correct Answer
B. False

Explanation
Using MALDI for AST (Antimicrobial Susceptibility Testing) cannot significantly improve susceptibility TAT (Turnaround Time) compared to traditional methods. This statement suggests that MALDI is not more efficient in providing results for AST than traditional methods.

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Current Version
Mar 21, 2023

Quiz Edited by
ProProfs Editorial Team
Oct 22, 2014

Quiz Created by
Nicole Siska

Bcid Training - Module 2 Quiz

MALDI can be performed after blood culture without a gram stain result.

Traditional blood culture with ID & AST typically takes an average of ____________ hours.

What technology is used in MALDI-TOF?

What is detected during the MALDI-TOF process?

MALDI-TOF results are analyzed by manually reading peaks of the spectrum.

What are the names of the most common MALDI-TOF Systems used in the US for blood culture?

A sample for MALDI-TOF testing can be performed directly from the blood culture bottle like molecular tests.

What is the average time to result to organism ID using a MALDI-TOF system?

The Bruker MALDI Biotyper is FDA cleared on gram-positive & gram-negative bacterial colonies cultured from human specimens. ​

Lysate preparation or extraction required for some bacteria and yeast is required on the Bruker Biotyper.

For each hour that appropriate targeted antimicrobial treatment is delayed, a sepsis patient’s mortality rate increases by ___%

Blood Culture ID/AST is commonly performed with MALDI.

Using MALDI for AST can significantly improve susceptibility TAT compared to traditional methods.

The Bruker MALDI Biotyper is FDA cleared on gram-positive & gram-negative bacterial colonies cultured from human specimens.