QUIZ ONLINE 3 CHAPTER 4 THE TOOLS OF THE TRADE

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Questions: 10 | Attempts: 162 | Updated: Mar 22, 2023

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Quiz Online 3 Chapter 4 The Tools Of The Trade - Quiz

Bekerja keras sekarang, merasakan hasilnya nanti; bermalas-malas sekarang, merasakan akibatnya nanti. -- John C. Maxwel

Questions and Answers

1.

Which of the following is NOT enzyme that used in gene manipulation?
- A.
  
  Primase
- B.
  
  Endonuclease
- C.
  
  Ligase
- D.
  
  Reverse transverase
Correct Answer
A. Primase

Explanation
Primase is not an enzyme used in gene manipulation. Primase is an enzyme involved in DNA replication, specifically in the synthesis of RNA primers that are necessary for DNA polymerase to initiate replication. In gene manipulation, enzymes such as endonucleases, ligases, and reverse transcriptase are commonly used. Endonucleases are used to cut DNA at specific sites, ligases are used to join DNA fragments together, and reverse transcriptase is used to convert RNA into DNA.

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2.

Restriction enzymes are named according to the bacterium from which they are purified.
- A.
  
  TRUE
- B.
  
  FALSE
Correct Answer
A. TRUE

Explanation
Enzymes are commonly named based on the bacterium they are purified from. This naming convention helps to identify and categorize enzymes based on their source, making it easier for researchers and scientists to study and understand their properties and functions. By naming enzymes after the bacterium they are derived from, it also provides information about the potential characteristics and behavior of the enzyme. Therefore, the statement that restriction enzymes are named according to the bacterium from which they are purified is true.

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3.

Enzymes which are purified from a wide range of organisms and can NOT be bought from various suppliers.
- A.
  
  TRUE
- B.
  
  FALSE
Correct Answer
B. FALSE

Explanation
Enzymes can be bought from various suppliers.

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4.

Which of the following is an enzyme that cuts DNA molecules at a limited number of specific nucleotide sequences?
- A.
  
  Reverse transverase
- B.
  
  DNA polymerase I
- C.
  
  Ligase
- D.
  
  Restriction endonuclease
Correct Answer
D. Restriction endonuclease

Explanation
Restriction endonuclease is an enzyme that cuts DNA molecules at specific nucleotide sequences. It recognizes and binds to specific DNA sequences, then cleaves the DNA at or near these recognition sites. This enzyme plays a crucial role in DNA manipulation and genetic engineering techniques, as it allows for the precise cutting and manipulation of DNA molecules. Reverse transverase, DNA polymerase I, and ligase are not involved in the process of cutting DNA at specific nucleotide sequences.

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5.

In essence the Restriction endonuclease may be thought of as molecular scissor.
- A.
  
  TRUE
- B.
  
  FALSE
Correct Answer
A. TRUE

Explanation
Restriction endonucleases are enzymes that can recognize specific DNA sequences and cut the DNA at those sites. This process is often referred to as molecular scissors because the enzyme acts like a pair of scissors cutting the DNA molecule at specific points. Therefore, the statement that the restriction endonuclease can be thought of as molecular scissors is true.

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6.

Determination of the positions of restriction sites in a DNA molecule by analyzing the sizes of restriction fragments.
- A.
  
  DNA mapping
- B.
  
  Genome mapping
- C.
  
  Gene mapping
- D.
  
  Restriction mapping
Correct Answer
D. Restriction mapping

Explanation
Restriction mapping is a technique used to determine the positions of restriction sites in a DNA molecule by analyzing the sizes of restriction fragments. Restriction sites are specific sequences of DNA where restriction enzymes can bind and cut the DNA. By digesting the DNA with different restriction enzymes and analyzing the resulting fragments using gel electrophoresis, the positions of the restriction sites can be determined. This information is useful in studying the structure and organization of DNA, as well as in identifying genes and regulatory elements.

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7.

Enzyme activity is expressed in units, with one unit being the amount of enzyme that will cleave one gram of DNA in one hour at 37^◦C.
- A.
  
  TRUE
- B.
  
  FALSE
Correct Answer
B. FALSE

Explanation
Enzyme activity is not expressed in units of cleaving DNA. Enzyme activity is typically measured in units of substrate converted or product formed per unit of time. The statement that enzyme activity is expressed in units of cleaving one gram of DNA in one hour at 37◦C is incorrect.

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8.

Which of the following is a polymerase that synthesizes DNA on an RNA template?
- A.
  
  Reverse transverase
- B.
  
  DNA polymerase I
- C.
  
  Ligase
- D.
  
  Restriction endonuclease
Correct Answer
A. Reverse transverase

Explanation
Reverse transcriptase is a polymerase that synthesizes DNA on an RNA template. It is an enzyme found in retroviruses and is responsible for the conversion of RNA into DNA during the process of reverse transcription. This enzyme plays a crucial role in the replication of retroviruses and is also used in molecular biology techniques such as reverse transcription polymerase chain reaction (RT-PCR) to convert RNA into complementary DNA (cDNA) for further analysis.

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9.

Which of the following is an enzyme that synthesizes phosphodiester bonds as part of DNA replication, repair and recombination processes?
- A.
  
  Reverse transverase
- B.
  
  DNA polymerase I
- C.
  
  Ligase
- D.
  
  Restriction endonuclease
Correct Answer
C. Ligase

Explanation
Ligase is an enzyme that plays a crucial role in DNA replication, repair, and recombination processes. It is responsible for synthesizing phosphodiester bonds between adjacent nucleotides, joining the sugar-phosphate backbone of DNA strands. This enzyme is essential for sealing the gaps that occur during DNA replication and repair, ensuring the integrity and stability of the DNA molecule. Ligase is not involved in reverse transcription (Reverse transverase), DNA synthesis (DNA polymerase I), or DNA cleavage (Restriction endonuclease).

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10.

An enzyme that degrades a nucleic acid molecule.
- A.
  
  Endonucleases
- B.
  
  Exonucleases
- C.
  
  Nuclease
- D.
  
  Ribonuclease
Correct Answer
C. Nuclease

Explanation
A nuclease is an enzyme that breaks down nucleic acid molecules. It can cleave the phosphodiester bonds within the nucleic acid backbone, resulting in the degradation of the molecule. Endonucleases specifically cleave the nucleic acid at internal sites, while exonucleases cleave at the ends of the molecule. Ribonucleases are a type of nuclease that specifically degrade RNA molecules. Therefore, the correct answer is nuclease, as it is a broad term encompassing all enzymes that degrade nucleic acid molecules.

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Current Version
Mar 22, 2023

Quiz Edited by
ProProfs Editorial Team
May 17, 2009

Quiz Created by
Faunsoed

Quiz Online 3 Chapter 4 The Tools Of The Trade

Which of the following is NOT enzyme that used in gene manipulation?

Restriction enzymes are named according to the bacterium from which they are purified.

Enzymes which are purified from a wide range of organisms and can NOT be bought from various suppliers.

Which of the following is an enzyme that cuts DNA molecules at a limited number of specific nucleotide sequences?

In essence the Restriction endonuclease may be thought of as molecular scissor.

Determination of the positions of restriction sites in a DNA molecule by analyzing the sizes of restriction fragments.

Enzyme activity is expressed in units, with one unit being the amount of enzyme that will cleave one gram of DNA in one hour at 37◦C.

Which of the following is a polymerase that synthesizes DNA on an RNA template?

Which of the following is an enzyme that synthesizes phosphodiester bonds as part of DNA replication, repair and recombination processes?

An enzyme that degrades a nucleic acid molecule.

Enzyme activity is expressed in units, with one unit being the amount of enzyme that will cleave one gram of DNA in one hour at 37^◦C.