Quiz | Real-Time PCR
After read through about real-time PCR in our website, we would like to invite you to take this quiz!
What after reading is understanding! Take this quiz now to see how far you understand about real-time PCR technique!
Start my 10 multiple choice questions.
All the best!
Sincerely from,
The authors (Ching Xin Huey & Yeo Zhin Leng)
Questions and Answers
- 1.
Who received the Nobel Prize in Chemistry in 1993 for inventing PCR?
- A.
David Baltimore
- B.
Kary Mullis
- C.
Robert Weinberg
- D.
John Nash
Correct Answer
B. Kary MullisExplanation
Kary Mullis received the Nobel Prize in Chemistry in 1993 for inventing PCR, which stands for Polymerase Chain Reaction. PCR is a technique used to amplify a specific segment of DNA, allowing scientists to study and analyze it in greater detail. Mullis' invention revolutionized the field of molecular biology and has since become an essential tool in various scientific disciplines, including genetics, forensics, and medical diagnostics.Rate this question:
-
- 2.
Nucleotide triphosphates (dNTPs) are added to the growing DNA strand during the _______________ phase.
- A.
Extension/elongation
- B.
Annealing
- C.
Denaturation/separation
- D.
Preparation
Correct Answer
A. Extension/elongationExplanation
During the extension/elongation phase of DNA replication, nucleotide triphosphates (dNTPs) are added to the growing DNA strand. This is when the DNA polymerase enzyme catalyzes the formation of phosphodiester bonds between the incoming nucleotides and the existing DNA strand, resulting in the elongation of the DNA molecule. This process ensures the accurate replication of the genetic information contained within the DNA molecule.Rate this question:
-
- 3.
What does the acronym PCR stand for?
- A.
Polymerase Chain Reaction
- B.
Polymerase Copying Reaction
- C.
Polymerase Copied Repeats
- D.
Polymerase Chain Restoration
Correct Answer
A. Polymerase Chain ReactionExplanation
PCR stands for Polymerase Chain Reaction. This technique is used to amplify a specific segment of DNA through a series of heating and cooling cycles. It involves the use of a DNA polymerase enzyme to copy and replicate the DNA region of interest. The process starts with denaturation, where the DNA strands are separated. Then, primers bind to the target DNA sequence, allowing the DNA polymerase to synthesize new DNA strands. This cycle is repeated multiple times, resulting in the exponential amplification of the desired DNA fragment. PCR is widely used in various applications, including genetic research, diagnostics, and forensic analysis.Rate this question:
-
- 4.
This is the sequence order of RT-PCR.1. Denature2. Anneal3. Detect4. Extend
- A.
True
- B.
False
Correct Answer
B. FalseExplanation
The correct answer is False. The sequence order of RT-PCR is as follows: 1. Denature, 2. Anneal, 3. Extend, and 4. Detect. This is the standard procedure for reverse transcription polymerase chain reaction, where the RNA template is first denatured to separate the strands, then the primers anneal to the target sequence, followed by extension of the primers by a DNA polymerase, and finally the detection of the amplified DNA product.Rate this question:
-
- 5.
Which is/are the property of real-time PCR assays?
- A.
Incorporate dyes that bind double-stranded DNA
- B.
Incorporate an internal hydrolysis probe
- C.
Be performed at single temperature with no specialized instrumentation required
- D.
Be interpreted as a plus / minus result or as a quantitative result
Correct Answer(s)
A. Incorporate dyes that bind double-stranded DNA
B. Incorporate an internal hydrolysis probe
D. Be interpreted as a plus / minus result or as a quantitative resultExplanation
Real-time PCR assays incorporate dyes that bind double-stranded DNA, which allows for the detection and quantification of the DNA target. They also incorporate an internal hydrolysis probe, which is a specific sequence of DNA that binds to the target DNA and releases a fluorescent signal during amplification. This probe helps in the accurate detection of the target DNA. Finally, the results of real-time PCR assays can be interpreted as either a plus/minus result (presence or absence of the target DNA) or as a quantitative result (measuring the amount of target DNA present).Rate this question:
-
- 6.
Choose the statement that correctly finishes the sentence:“A PCR reaction that contains only one copy of the target sequence (1 copy /reaction)…
- A.
Is typically amplified in a highly repeatable manner”
- B.
May amplify but is detection is not likely to be highly repeatable”
- C.
Can be precisely and accurately quantified using quantitative PCR”
- D.
All of the above
Correct Answer
B. May amplify but is detection is not likely to be highly repeatable”Explanation
A PCR reaction that contains only one copy of the target sequence may amplify, but its detection is not likely to be highly repeatable. This is because when there is only one copy of the target sequence, there is a higher chance of experimental variability and errors in the detection process. Therefore, although amplification may occur, the results may not be consistently reproducible.Rate this question:
-
- 7.
RNA is copied into complementary DNA (cDNA) by:
- A.
Taq DNA polymerase
- B.
RNA polymerase II
- C.
Reverse transcriptase
- D.
Uracil-N-Glycosylase
Correct Answer
C. Reverse transcriptaseExplanation
Reverse transcriptase is an enzyme that is responsible for the synthesis of complementary DNA (cDNA) from an RNA template. It is commonly used in molecular biology techniques such as reverse transcription polymerase chain reaction (RT-PCR) to convert RNA into DNA. This enzyme is derived from retroviruses and is capable of catalyzing the reverse transcription of RNA into DNA. Therefore, it is the correct answer for the given question.Rate this question:
-
- 8.
The reverse transcriptase reaction can be primed by
- A.
Target sequence specific primers
- B.
Random hexamers
- C.
Oligo dT primers
- D.
All of the above
Correct Answer
D. All of the aboveExplanation
In the reverse transcriptase reaction, primers are used to initiate the synthesis of cDNA from RNA. These primers can be target sequence specific, meaning they are designed to bind to a specific region of the RNA molecule. They can also be random hexamers, which are short DNA sequences that bind randomly to the RNA template. Additionally, oligo dT primers, which are composed of a string of thymine bases, can be used to bind to the poly-A tail found on most mRNA molecules. Therefore, all of the given options - target sequence specific primers, random hexamers, and oligo dT primers - can be used to prime the reverse transcriptase reaction.Rate this question:
-
- 9.
Select the application of RT-PCR.
- A.
Drug therapy efficacy
- B.
Verification of microarray results
- C.
Allelic discrimination assays or SNP genotyping
- D.
Quantitative mRNA expression studies
- E.
DNA replication efficiency
Correct Answer(s)
A. Drug therapy efficacy
B. Verification of microarray results
C. Allelic discrimination assays or SNP genotyping
D. Quantitative mRNA expression studiesExplanation
RT-PCR (Reverse Transcription Polymerase Chain Reaction) is a technique used to amplify and detect specific RNA molecules. It is commonly used in various applications such as drug therapy efficacy, verification of microarray results, allelic discrimination assays or SNP genotyping, and quantitative mRNA expression studies. In drug therapy efficacy, RT-PCR can be used to determine the effectiveness of a drug by measuring the expression levels of specific genes. In verification of microarray results, RT-PCR can be used to validate the gene expression data obtained from microarray experiments. In allelic discrimination assays or SNP genotyping, RT-PCR can be used to detect specific genetic variations. In quantitative mRNA expression studies, RT-PCR can be used to measure the expression levels of specific mRNA molecules.Rate this question:
-
- 10.
Human errors limits the accuracy of the real-time PCR results:
- A.
Incorrect data analysis
- B.
improper assay development
- C.
Destroy of DNA
- D.
Unwarranted conclusions
Correct Answer(s)
A. Incorrect data analysis
B. improper assay development
D. Unwarranted conclusionsExplanation
Human errors can limit the accuracy of real-time PCR results in several ways. Incorrect data analysis can lead to misinterpretation of the results and incorrect conclusions. Improper assay development, such as using incorrect primer sequences or suboptimal reaction conditions, can result in inaccurate measurements. Additionally, unwarranted conclusions can be drawn if proper controls and validation steps are not followed, leading to false interpretations of the data. These human errors highlight the importance of careful and meticulous experimental design, execution, and analysis in real-time PCR to ensure accurate and reliable results.Rate this question:
-
Quiz Review Timeline +
Our quizzes are rigorously reviewed, monitored and continuously updated by our expert board to maintain accuracy, relevance, and timeliness.
-
Current Version
-
Mar 20, 2023Quiz Edited by
ProProfs Editorial Team -
Mar 05, 2018Quiz Created by
Mohammed Naseruddin
Quiz: How Well Do You Know Different Science Careers?
Quiz: How Well Do You Know Different Science Careers?
Carl Linnaeus Quiz: Are You a True Carl Linnaeus Fan?
Carl Linnaeus Quiz: Are You a True Carl Linnaeus Fan?
Ada Lovelace Day Quiz: Do You Know About Women in Science?
Ada Lovelace Day Quiz: Do You Know About Women in Science?
Magnetic Particle Testing: Principles and Practice Quiz
Magnetic Particle Testing: Principles and Practice Quiz
General Science - General Knowledge questions and answers
General Science - General Knowledge questions and answers
- Aeronautics Quizzes
- Aerospace Quizzes
- Agricultural Science Quizzes
- Astrology Quizzes
- Astronomy Quizzes
- Atom Quizzes
- Biochemistry Quizzes
- Biology Quizzes
- Biomechanics Quizzes
- Biostatistics Quizzes
- Biotechnology Quizzes
- Botany Quizzes
- Branches Of Science Quizzes
- Chemistry Quizzes
- Cytology Quizzes
- Easy Science Quizzes
- Ecology Quizzes
- Electrical Quizzes
- Embryology Quizzes
- Endocrinology Quizzes
- Engineering Quizzes
- Environmental Science Quizzes
- Epidemiology Quizzes
- Experiment Quizzes
- Forestry Quizzes
- Fossil Quizzes
- Gas Quizzes
- General Science Quizzes
- Genetics Quizzes
- Histology Quizzes
- Human Biology Quizzes
- Integrated Science Quizzes
- Invention Quizzes
- Library Science Quizzes
- Lighting Quizzes
- Liquid Quizzes
- Marine Biology Quizzes
- Microbiology Quizzes
- Molecular Biology Quizzes
- Nature Quizzes
- Neuroscience Quizzes
- Nuclear Science Quizzes
- Oceanography Quizzes
- Physics Quizzes
- Psychology Quizzes
- Science And Technology Quizzes
- Science Glossary Quizzes
- Science Knowledge Quizzes
- Science Practice Quizzes
- Scientific Method Quizzes
- Scientific Notation Quizzes
- Soil Science Quizzes
- Solar System Quizzes
- Solid Quizzes
- Zoology Quizzes
Back to top